A proteomic analysis of liver after ethanol binge in chronically ethanol treated rats

Table 1 Identification of differentially expressed protein spots by mass spectrometry

Spot No.	Identification^a	Accession No.	MW (Da)^b	Total Ion Score^c	No. peptides matched
63	Carbonic anhydrase 3	3137484	29698	206	7
64	Carbonic anhydrase 3	3137484	29698	505	12
213	Cytosolic isocitrate dehydrogenase^d	6647554	47030	202	10
213	Glutamine synthetase^d	204349	43001	133	5
53	Glutathione S transferase M1	204503	26127	674	17
116	Protein disulfide isomerase A3	1352384	57044	163	6
117	Protein disulfide isomerase A3	1352384	57044	797	22
120	Protein disulfide isomerase A3	1352384	57044	994	28
123	Protein disulfide isomerase A3	1352384	57044	1294	30

^aData from LCMS runs were searched against NCBInr limited to mammals using MASCOT V2.2.
^bThis is the predicted molecular weight based on the translated gene sequence.
^cThe total ion score from the MASCOT report. Individual peptide ion scores >36 have significant identify or homology (p < 0.05).
^dTwo proteins were confidently identified in spot number 213. Based on number of peptides matched, isocitrate dehydrogenase is likely the more abundant of the two.

ISSN: 1477-5956