Proteomic analysis of purified Newcastle disease virus particles

Ren, Xiangpeng; Xue, Chunyi; Kong, Qingming; Zhang, Chengwen; Bi, Yingzuo; Cao, Yongchang

doi:10.1186/1477-5956-10-32

Proteome Science

Table 1 Virus-encoded structural proteins and cellular proteins associated with purified NDV particles identified by MALDI-TOF/TOF MS

From: Proteomic analysis of purified Newcastle disease virus particles

Spot no.^a	Protein description^b	Protein Funtion	Accession no.^c	Theoretical MW^d/pI^e	Score^f	Pep. no.^g	Reported in other viruses
A	NDV NP	Viral protein	AAC28371	53047/5.29	455	19
B	NDV P	Viral protein	AAC28372	42383/6.01	582	17
C	NDV M	Viral protein	AAC28373	39538/9.58	149	8
D	NDV F	Viral protein	AAC28374	58896/8.56	232	7
E	NDV HN	Viral protein	AAC28376	63213/7.13	105	6
1	S100 calcium-binding protein A6	Calcium ion binding	IPI00572547	10269.3/4.91	125	8	IBV[37]
2	S100 calcium-binding protein A11	Calcium ion binding	IPI00599279	11405.8/6.08	98	6	IV[35], VV[25, 26], EBV[19], HCMV[17], KSHV[20, 21], VSV[36], IBV[37]
3	aldolase A	Metabolic process	IPI00583507	14429.2/6.13	214	4	PRRSV[38]
4	Ras small GTPase	Signal transduction	IPI00683096	18292.1/4.64	76	7
5	tumor protein, translationally-controlled 1	Calcium ion binding	IPI00821768	19517.6/4.9	95	3
6	tumor protein D52	Calcium ion binding	IPI00579134	19848/4.94	114	5
7	YWHAE 14-3-3, zeta polypeptide	Enzyme binding	IPI00820692	23944.1/5.23	211	6	IBV[37]
8	chromatin modifying protein 5	chromatin modifying	IPI00589508	24586.4/4.74	136	4	KSHV[39]
9	chromatin modifying protein 2A	chromatin modifying	IPI00594797	24716.8/5.57	158	5	KSHV[39]
10	chromatin modifying protein 4B	chromatin modifying	IPI00582041	25139.7/4.73	196	6	KSHV[39]
11	chromatin modifying protein 4 C	chromatin modifying	IPI00580681	25145.6/5.5	95	7	KSHV[39]
12	YWHAE 14-3-3, theta polypeptide	Enzyme binding	IPI00577739	27764.7/4.68	89	3	IBV[37]
13	nucleoporin 210kDaaa-like	Uncharactered	IPI00813608	28743.7/4.69	253	7
14	YWHAE 14-3-3, epsilon polypeptide	Enzyme binding	IPI00579092	28944.4/4.75	162	4	IBV[37]
15	syntenin;syndecan binding protein	Signal transduction	IPI00598186	32037.6/7.01	187	6
16	tropomyosin 1 alpha	cytoskeleton	IPI00600961	32482.6/4.68	93	5	HIV[31, 32], IV[35]
17	capping protein muscle Z-line, alpha 2	cytoskeleton	IPI00683096	32964.7/6.34	86	7	HIV[31, 32], IV[35]
18	Annexin A8-like 1	Uncharactered	IPI00585409	36709.9/5.24	153	10
19	Annexin A2	cytoskeleton	IPI00577039	38615.9/6.92	172	12	HCMV[17], HIV[31, 32], SARS [33], IV[35], IBV[37], PRRSV[38], herpes simplex virus 1[40]
20	KIAA0174	cytoskeleton	IPI00581393	39714.6/5.22	106	6	PRRSV[38]
21	Actin, gamma 1 propeptide;	cytoskeleton	IPI00572084	41808.8/5.3	453	12	HIV[31, 32], SARS [33, 34], IV[35], VV[25, 26], EBV[19], HCMV[17], KSHV[20, 21], VSV[36], IBV[37], PRRSV[38]
22	ovalbumin	Metabolic process	IPI00583974	42853.5/5.19	269	10	IBV[37]
23	ARP2 Actin -related protein 2 homolog	Uncharactered	IPI00585509	44673.3/6.3	219	8
24	ARP3 Actin -related protein 3	cytoskeleton	IPI00587398	47391.1/5.62	206	7	IBV[37]
25	similar to type I hair keratin KA31	Uncharactered	IPI00587107	51130.7/4.78	91	4
26	Ezrin	cytoskeleton	IPI00578484	69323.9/5.9	135	6	HIV[31, 32], IV[35]
27	Heat shock protein 70	Molecular chaperone	IPI00818704	70955.3/5.43	140	4	HIV[31, 32], SARS [33, 34], IV[35], VSV[36], IBV[37], PRRSV[38], Adenovirus[41], enterovirus[42], vaccinia virus[43], hantaan virus[44]
28	CAP-GLY containing linker protein 2	cytoskeleton	IPI00579643	116233.3/6.45	81	3	IBV[37]
29	Tubulin, alpha-1	cytoskeleton	IPI00591483	49639/4.78	259	6	HIV[31, 32], SARS [33, 34], IV[35], VV[25, 26], EBV[19], HCMV[17], KSHV[20, 21], VSV[36], IBV[37], PRRSV[38]
30	heat shock 90 kDa aa protein 1, alpha	Molecular chaperone	IPI00596586	84006.5/5.01	130	7	IBV[37]

a Protein spot numbers on 2-DE gel.
b Alternative names are provided in parentheses.
c Accession numbers ford NCBI database (accessible at http://www.ncbi.nlm.nih.gov/).
d Theoretical molecular mass.
e Theoretical pI.
f MASCOT protein score (based on combined MS and MS/MS spectra) of greater than 64 (p ≤ 0.05) or the total ion score (based on MS/MS spectra) of greater than 30 (p ≤ 0.05).
g Observed peptides that differ either by sequence, modification or charge.

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ISSN: 1477-5956

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