Potential plasma biomarkers for progression of knee osteoarthritis using glycoproteomic analysis coupled with a 2D-LC-MALDI system

Table 1 Biomarker candidates identified by proteomic analysis

Peak cluster (sample set)	Correlation coefficient (r)*	Fold change**	P value***	Identified peptide	Protein name	Accession No.	Mascot score	Error, Da (ppm)
2932 (#1)	0.799	6.4	0.012	LVPVPITN AT LDRITGK	α-1 acid glycoprotein-2	NP_000599	98	0.131 (72.4)
2424 (#2)	0.769	2.9	0.007	LVPVPITN AT LDRITGK	α-1 acid glycoprotein-2	NP_000599	98	0.131 (72.4)
4512 (#1)	0.894	2.3	0.199	MLN TS SLLEQLNEQFNWVSR	Clusterin	NP_976084	66	0.052 (21.6)
3819 (#2)	0.812	3.5	0.009	MLN TS SLLEQLNEQFNWVSR	Clusterin	NP_976084	66	0.052 (21.6)
4878 (#1)	0.916	3.1	0.028	GTGN DT VLNVALLNVISNQECNIK	Macrophage stimulating protein	NP_066278	43	0.221 (85.3)
4126 (#2)	0.720	2.1	0.061	GTGN DT VLNVALLNVISNQECNIK	Macrophage stimulating protein	NP_066278	43	0.221 (85.3)
4483 (#1)	0.731	19.3	0.005	GHGHRN GT GHGN ST HHGPEYMR	Hemopexin	NP_000604	96	0.118 (49.0)
3797 (#2)	0.916	10.0	0.007	GHGHRN GT GHGN ST HHGPEYMR	Hemopexin	NP_000604	96	0.118 (49.0)

N-Glycosylation motifs (N-X-S/T) are indicated in bold, and glycosylated asparagine residues are underlined.
*Correlation coefficient between the peak intensity and the rate of JSW narrowing. **Calculated from the mean intensity of progressors (n = 3) to that of non-progressors from respective sample set (#1, #2). ***Calculated by Student’s t-test.

ISSN: 1477-5956