Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

Table 2 Co-migration of iTRAQ- and unlabelled peptides in the same OGE fraction

	Sequence	Mass NL	Mass L	Difference	F NL	F L
1	MFDAGLYEHCR*	1397.58	1542.69	145.12	4	4
2	EAFPGDVFYLHSR*	1536.75	1681.85	145.09	4	5
3	TLHGLQPPESSGIFNEK*	1852.93	2142.14	289.22	4	4
4	ATFDCLMK*	984.49	1273.65	289.16	5	5
5	GIPYLNTYDGR*	1267.67	1412.73	145.06	5,6	5,6
6	GFGFVTFANEK	1215.62	1504.80	289.18	2,6	6
7	GKDFAELIASGR	1262.67	1551.87	289.21	6	6
8	AALNDFDRFK	1195.60	1484.81	289.21	6	6
9	EAQWAHAQR*	1095.52	1240.63	145.11	8	8
10	SRFFHSTGQR*	1221.54	1366.71	145.17	8	8
11	AGDFFHSAQSR*	1221.56	1366.66	145.11	8	8
12	ASALIQHDWSR*	1282.63	1427.75	145.12	8	8
13	AHGGFSVFAGVGER	1389.69	1534.79	145.10	8	8
14	VGPFHNPSETYR	1402.65	1547.77	145.12	8	8
15	GVDKEHVMLLAAR	1437.77	1726.99	289.22	8	8
16	EVHFLPFNPVDKR	1596.85	1886.05	289.20	8	8
17	EIHFLPFNPVDKR	1610.87	1900.07	289.20	8	8
18	ALYHDLNAYR	1234.61	1379.72	145.11	8	8
19	AGVKPHELVF	1095.61	1384.82	289.21	8	8
20	LAWHSAGTFDSK*	1318.61	1607.84	289.23	8	8
21	YDTVHGQWK	1132.52	1421.74	289.22	8	8
22	NGGANFVAPGYTK	1294.54	1583.84	289.31	10	10
23	AASFNIIPSSTGAAK	1433.76	1722.96	289.21	10	10
24	FVTAVVGFGK	1023.57	1312.79	289.21	10	10
25	AGQYNFLIR	1080.58	1225.68	145.10	12	12
26	AYGGVLSGGAVR	1105.59	1250.70	145.10	12	12
27	GFQTSYYNR	1134.50	1279.62	145.12	11,12	12
28	VLNTGSPISVPVGR	1394.76	1539.90	145.13	12	12
29	SSSVFIPHGPGAVR	1409.73	1554.85	145.12	12	12
30	LVSAHSSQQIYTR	1488.75	1633.88	145.13	12	12
31	GGGHTSQIYAIR	1258.65	1403.75	145.11	12	12
32	HGSLGFLPR	982.53	1127.64	145.12	12	12
33	GGQLIYGGPLGR	1186.63	1331.76	145.12	12	12
34	AGGAYTLNTASAVTVR	1550.79	1695.91	145.12	12	12

Thirty-four peptides focused in the same OGE fraction in both label-free and iTRAQ labelled experiments are shown in this table. Peptides shifted to more basic fractions in at least one experiment are indicated with an asterisk (*).

ISSN: 1477-5956