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Table 2 Co-migration of iTRAQ- and unlabelled peptides in the same OGE fraction

From: Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

  Sequence Mass NL Mass L Difference F NL F L
1 MFDAGLYEHCR* 1397.58 1542.69 145.12 4 4
2 EAFPGDVFYLHSR* 1536.75 1681.85 145.09 4 5
3 TLHGLQPPESSGIFNEK* 1852.93 2142.14 289.22 4 4
4 ATFDCLMK* 984.49 1273.65 289.16 5 5
5 GIPYLNTYDGR* 1267.67 1412.73 145.06 5,6 5,6
6 GFGFVTFANEK 1215.62 1504.80 289.18 2,6 6
7 GKDFAELIASGR 1262.67 1551.87 289.21 6 6
8 AALNDFDRFK 1195.60 1484.81 289.21 6 6
9 EAQWAHAQR* 1095.52 1240.63 145.11 8 8
10 SRFFHSTGQR* 1221.54 1366.71 145.17 8 8
11 AGDFFHSAQSR* 1221.56 1366.66 145.11 8 8
12 ASALIQHDWSR* 1282.63 1427.75 145.12 8 8
13 AHGGFSVFAGVGER 1389.69 1534.79 145.10 8 8
14 VGPFHNPSETYR 1402.65 1547.77 145.12 8 8
15 GVDKEHVMLLAAR 1437.77 1726.99 289.22 8 8
16 EVHFLPFNPVDKR 1596.85 1886.05 289.20 8 8
17 EIHFLPFNPVDKR 1610.87 1900.07 289.20 8 8
18 ALYHDLNAYR 1234.61 1379.72 145.11 8 8
19 AGVKPHELVF 1095.61 1384.82 289.21 8 8
20 LAWHSAGTFDSK* 1318.61 1607.84 289.23 8 8
21 YDTVHGQWK 1132.52 1421.74 289.22 8 8
22 NGGANFVAPGYTK 1294.54 1583.84 289.31 10 10
23 AASFNIIPSSTGAAK 1433.76 1722.96 289.21 10 10
24 FVTAVVGFGK 1023.57 1312.79 289.21 10 10
25 AGQYNFLIR 1080.58 1225.68 145.10 12 12
26 AYGGVLSGGAVR 1105.59 1250.70 145.10 12 12
27 GFQTSYYNR 1134.50 1279.62 145.12 11,12 12
28 VLNTGSPISVPVGR 1394.76 1539.90 145.13 12 12
29 SSSVFIPHGPGAVR 1409.73 1554.85 145.12 12 12
30 LVSAHSSQQIYTR 1488.75 1633.88 145.13 12 12
31 GGGHTSQIYAIR 1258.65 1403.75 145.11 12 12
32 HGSLGFLPR 982.53 1127.64 145.12 12 12
33 GGQLIYGGPLGR 1186.63 1331.76 145.12 12 12
34 AGGAYTLNTASAVTVR 1550.79 1695.91 145.12 12 12
  1. Thirty-four peptides focused in the same OGE fraction in both label-free and iTRAQ labelled experiments are shown in this table. Peptides shifted to more basic fractions in at least one experiment are indicated with an asterisk (*).