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Table 3 Acidic pI shifts in OGE as a result of iTRAQ labelling

From: Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

  Sequence Mass NL Mass L Difference F NL F L
1 SKFDNLYGCR 1258.56 1547.79 289.22 11 10
2 QFNGLVDVYKK$ 1292.69 1743.02 450.33 11 9,10
3 KGPLIVYGTEGAK 1331.73 1765.06 433.33 11 9
4 YLQPQESGWKPK 1459.80 1893.06 433.26 11 9,10
5 GVQQVLQNYK 1175.61 1464.84 289.23 12 10
6 KQFVIDVLHPGR 1407.81 1697.01 289.20 12 10
7 ASALIQHEWRPK 1434.76 1723.98 289.22 12 10
8 LSEPYKGIGDCFKR 1668.82 2102.14 433.33 12 9,10
9 GVLPQNQPFVVK 1324.74 1613.96 289.22 12 10,11
10 INWLTNPVHK 1220.65 1509.88 289.22 12 10
11 IAGFSTHLMK 1103.57 1392.79 289.22 12 10
  1. Eleven peptides shifted to more acidic OGE fraction after iTRAQ labelling are presented in this table.
  2. $Gln- > pyro-Glu@N-term.