Optimization of iTRAQ labelling coupled to OFFGEL fractionation as a proteomic workflow to the analysis of microsomal proteins of Medicago truncatula roots

Table 3 Acidic pI shifts in OGE as a result of iTRAQ labelling

	Sequence	Mass NL	Mass L	Difference	F NL	F L
1	SKFDNLYGCR	1258.56	1547.79	289.22	11	10
2	QFNGLVDVYKK^$	1292.69	1743.02	450.33	11	9,10
3	KGPLIVYGTEGAK	1331.73	1765.06	433.33	11	9
4	YLQPQESGWKPK	1459.80	1893.06	433.26	11	9,10
5	GVQQVLQNYK	1175.61	1464.84	289.23	12	10
6	KQFVIDVLHPGR	1407.81	1697.01	289.20	12	10
7	ASALIQHEWRPK	1434.76	1723.98	289.22	12	10
8	LSEPYKGIGDCFKR	1668.82	2102.14	433.33	12	9,10
9	GVLPQNQPFVVK	1324.74	1613.96	289.22	12	10,11
10	INWLTNPVHK	1220.65	1509.88	289.22	12	10
11	IAGFSTHLMK	1103.57	1392.79	289.22	12	10

Eleven peptides shifted to more acidic OGE fraction after iTRAQ labelling are presented in this table.
^$Gln- > pyro-Glu@N-term.

ISSN: 1477-5956