Figure 2
From: A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
Verification of PM purification through Western blotting. 50 micrograms of homogenization and PM proteins were separated in 11.5% SDS-PAGE and then transferred to a PVDF membrane. The blots were probed with antibodies against organelle-specific proteins: anti-Na+/K+-ATPase for PM; anti-prohibitin for mitochondria. A9, N9 represent liver plasma membrane proteins from 9-weeks alcohol treated rat models and the control group. Homo means homogenization proteins from the liver of normal rat at 9 weeks.