Figure 5
From: A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis
Western blotting analyses of selected proteins. 50 μg PM Protein extracts of 2, 6 and 9 weeks were separated by 11.5% separation gels and then transferred to a PVDF membrane. The blots were probed with antibodies against ANX3 and ANX6. A, The expression of ANX3 and ANX6 in 6 and 9 weeks rat model. ALF-2 and Nor-2 represent liver plasma membrane proteins from 2-weeks alcohol treated rat models and the control group. B, The signal intensity of specific bands of ANX3 analyzed by ImageJ software. C, The signal intensity of specific bands of ANX6. The data were presented as average value (from a protein loading of 50 ug) and standard deviation (n = 3).