Figure 2

Screening an ORFV expression library for genes encoding products that direct accumulation of MHCII-positive cells. Left panels. Light microscope view pool-inoculated tissue sections, showing the distribution and density of gold microparticles delivered to cells by gene gun. Right panels. Tissue sections prepared from the same pool-inoculated mouse skin shown in left panel were stained with FITC-labeled antibody 2G9 (anti- I-A^d/I-E^d) to visualize I-A⁺/I-E⁺ immune cells. Each panel shows skin samples obtained 4 days after gene gun inoculation with pools of mixed-ORFs (2 μg of total pool DNA per site). Tissue staining densities within sections harvested from mice inoculated with pools I, J, and K are representative of all but X. The sections obtained from the mice shot with pool X displayed an increase in staining. Scale bars are 200 micron.