Figure 2

iTRAQ experimental design. Duplicates of wild-type cells that were starved of glucose were harvested. Samples were processed, digested with trypsin, and the resulting peptides were labeled with 113 and 114. Duplicates of cells that were glucose-starved and then transferred to medium containing glucose for 2 hours were harvested. Samples were processed and digested with trypsin. The resulting tryptic digested peptides were labeled with 115 and 116. The 113/114 and 115/116 ratios were used as control ratios (replicates in one MS run). The ratios of 115/113, 115/114, 116/113, and 116/114 were used as re-fed/starved ratios.