Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

He, Lizhong; Lu, Xiaomin; Tian, Jing; Yang, Yanjuan; Li, Bin; Li, Jing; Guo, Shirong

doi:10.1186/1477-5956-10-42

Proteome Science

Table 1 Differentially expression proteins identified by MALDI-TOF/TOF MS

From: Proteomic analysis of the effects of exogenous calcium on hypoxic-responsive proteins in cucumber roots

^aSpot No.	Protein name	Groups	Accession No.	Plant species	^bMr/P I	Score	^cPM	^eCov (%)	^fprotein expression (%Vol)
									A B C
1	JGCCJG2048B02.b Jatropha curcas L. germinating seeds (mixed stages) Jatropha curcas cDNA clone	EST sequence	gi\|302362663	Jatropha curcas	26.6/7.26	135	4	6.27
2	Unnamed protein product	Other	gi\|9759529	Arabidopsis thaliana	132.67/5.4	78	23	16.90
4	Aconitate hydratase, cytoplasmic	Citric acid cycle	gi\|1351856	Cucurbita maxima (winter squash)	98.57/5.74	184	16	19.04
5	V-type proton ATPase catalytic subunit A	Energy metabolism	gi\|401322	Gossypium hirsutum (upland cotton)	68.76/5.36	270	19	33.87
8	phosphoglycerate mutase	Glycolysis	gi\|32400802	Triticum aestivum (bread wheat)	29.62/5.43	169	8	30.43
10	Enolase	Glycolysis	gi\|14423688	Hevea brasiliensis	48.0/5.57	180	8	21.75
11	Enolase	Glycolysis	gi\|1169534	Ricinus communis (castor bean)	48.1/5.56	266	10	25.84
12	starch synthase III	Energy metabolism	gi\|9502143	Triticum aestivum (bread wheat)	184.0/4.94	68	22	12.10
13	TransId-212581 CACATN1 Coffea arabica cDNA clone	EST sequence	gi\|257024642	Coffea arabica	32.1/10.27	87	12	12.31
14	CBOZ5962.b1 CBOZ Coccomyxa sp. C-169 8 kb Coccomyxa sp. C-169 cDNA clone	EST sequence	gi\|282500599	Coccomyxa subellipsoidea C-169	25.4/10.03	85	12	16.27
15	CLS_cLiFproots_25a3_1_h11cLibkit5LD_D06 CLS_cLiFproots_plant Festuca arundinacea cDNA clone	EST sequence	gi\|257183562	Festuca arundinacea	39.4/9.76	88	13	10.27
16	cofactor-independent phosphoglyceromutase	Glycolysis	gi\|6706331	Apium graveolens	61.1/5.26	112	7	12.27
17	putative protein phosphatase 2 C	Protein synthesis	gi\|50725575	Oryza sativa Japonica Group	34.6/4.88	66	4	14.15
18	galactokinase	Glycolysis	gi\|53747925	Pisum sativum	55.2/5.4	78	3	5.84
19	Glutamine synthetase cytosolic isozyme	Nitrogen metabolism	gi\|12643762	Lotus japonicus	39.3/5.49	95	7	24.44
20	pyruvate dehydrogenase2	Citric acid cycle	gi\|162464059	Zea mays	40.1/5.54	184	5	12.06
22	Malate dehydrogenase, cytoplasmic	Citric acid cycle	gi\|11133373	Medicago sativa	35.9/6.39	624	11	48.49
24	CLS_cLiFproots_52a4_1_b18cLibkit5LD_ A09 CLS_cLiFproots_plant Festuca arundinacea cDNA clone	EST sequence	gi\|257180604	Festuca arundinacea	41.9/10.49	100	14	11.14
25	Os03g0851100	Other	gi\|115456623	Oryza sativa Japonica Group	48.6/6.04	77	7	15.45
26	alcohol dehydrogenase	Fermentative metabolism	gi\|52851054	Populus tremula	33.1/6.08	173	7	24.44
27	putative pyruvate dehydrogenase e1 alpha subunit	Citric acid cycle	gi\|13430788	Arabidopsis thaliana	43.5/7.15	178	13	34.20
28	methionyl-tRNA synthetase	Protein synthesis	gi\|4091008	Oryza sativa (rice)	90.9/6.55	78	15	18.66
30	fructose-bisphosphate aldolase, class I	Glycolysis	gi\|15227981	Arabidopsis thaliana	38.7/7.01	282	8	27.37
31	peroxidase	Defense against stress	gi\|167531	Cucumis sativus (cucumber)	32.7/6	96	2	5.78
32	Os02g0121900	Other	gi\|115443885	Oryza sativa Japonica Group	70.3/9.39	73	14	28.69
33	F1-ATP synthase, beta subunit	Energy metabolism	gi\|4388533	Sorghum bicolor	49.2/5.25	898	16	45.81
34	Os06g0597200	Other	gi\|115468776	Oryza sativa Japonica Group	40.1/5.32	78	6	12.66
36	putative fructokinase	Glycolysis	gi\|14423528	Arabidopsis thaliana	35.4/5.3	221	4	13.54
37	FS080420 library SmFL Solanum melongena cDNA clone	EST sequence	gi\|261665622	Solanum melongena	21.2/10.18	88	12	19.02
38	GSTSUB_UP_031_F12_01SEP2004_086 GSTSUB Artemisia annua cDNA, mRNA sequence	EST sequence	gi\|283968778	Artemisia annua	23.1/10.1	106	11	14.81

^aSpot numbers are given in Figure 1.
^bTheoretical molecular mass (Mr) and isoelectric point (pI) of the identified proteins.
^eThe percent coverage of peptides.
^fhe relative levels of protein expression. A: normoxic; B: hypoxia; C hypoxia + CaCl₂.

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ISSN: 1477-5956

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