Figure 5

Rosuvastatin-induced S-nitrosylation of vimentin and tropomyosin. Forty micrograms of biotinylated lysates derived from the biotin switch method were liquot and separated by SDS-PAGE following western blotting with antibodies against vimentin (VIM) and tropomyosin (TPM). These lysates were assigned as “before pull-down” (PD). The other biotinylated lysates (~500 μg) were incubated with 10 μl neutravidin-agarose to PD biotinylated proteins and assigned as “after PD”. The pulled-down lysates then underwent western blotting with the same antibodies. The relative fold “before PD” and “after PD” changes are shown by mean ± S.E. compared with control treatment. *p < 0.01 from three separate experiments by using Fisher’s LSD.