Table 3 Unique fragment ions used to quantify phosphorylation sites
Start | Stop | Peptide sequence | Phosphorylation site | Product ion ( m/z )* | Mass (Da) |
|---|---|---|---|---|---|
28 | 38 | GpSLTEQEPAER | Ser29 | y8 (+1) | 959.40 |
y9 (+1) | 1072.50 | ||||
501 | 511 | KLNpSTSDIEEK | Ser504 | b4 (+1) | 523.23 |
y7 (+1) | 821.39 | ||||
645 | 659 | pSpTQGVTLTDLQEAER | Ser645/Thr646 | b2 (+1) | 269.05 |
b3 (+1) | 397.11 | ||||
b4 (+1) | 454.13 | ||||
b5 (+1) | 553.20 | ||||
y10 (+1) | 1175.59 | ||||
y11 (+1) | 1274.66 | ||||
y12 (+1) | 1331.68 | ||||
y13 (+1) | 1459.74 | ||||
711 | 720 | pSLDEEPICHR | Ser711 | y6 (+1) | 754.37 |
y7 (+1) | 883.41 | ||||
y8 (+1) | 998.44 | ||||
755 | 773 | FSVPDpSESSETTTNTTTAK | Ser760† | b7 (+1) | 842.30 |
y12 (+1) | 1241.59 | ||||
y13 (+1) | 1370.63 | ||||
837 | 853 | LEpSGGSNPTTSDSYGDR | Ser839 | b3 (+1) | 410.10 |
y12 (+1) | 1299.50 | ||||
y13 (+1) | 1356.60 | ||||
y14 (+1) | 1413.60 |