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Figure 2 | Proteome Science

Figure 2

From: Changes in the expression of proteins associated with aerobic glycolysis and cell migration are involved in tumorigenic ability of two glioma cell lines

Figure 2

Functional analysis of cell lineages and expression of GRP78. A) The U87MG cell line shows a higher proliferation rate than the T98G cell line. Cells were counted in a Neubauer chamber at 24 h intervals; B) Thapsigargin decreased tumor cell migration. A transwell migration assay was carried out in U87MG and T98G cells after 18 h of incubation with or without 1 μM thapsigargin. Cells that migrated to the low chamber were fixed, stained, and counted as described in Materials and Methods. C) Thapsigargin increased GRP78 expression. Western blot analysis with anti-GRP78 antibody was carried out in both cell lines after 24 h of incubation with 1 μM thapsigargin. Expression of β-actin in cell lysates was used as a control.

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Proteome Science

ISSN: 1477-5956