Proteomic identification of MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana

Guo, Jing; Pang, Qiuying; Wang, Lihua; Yu, Ping; Li, Nan; Yan, Xiufeng

doi:10.1186/1477-5956-10-57

Proteome Science

Table 1 Protein identities and relative changes in their expression between MeJA-treated jin1-9/myc2 and wild-type plants after 6 h or 48 h of MeJA treatment

From: Proteomic identification of MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana

Spot No. ^a	Category and name	gi Number ^b	Exp pI/kDa ^c	Thero pI/kDa ^d	Score ^e	SC ^f (%)	PN ^g	MeJA-Treated WT 6h	MeJA-Treated jin1-9 6h ^h	Fold change	*p-value*	MeJA-Treated WT 48h	MeJA-Treated jin1-9 48h ^h	Fold change	*p-value*
								Vol% (mean±SD)	Vol% (mean±SD)			Vol% (mean±SD)	Vol% (mean±SD)
	Glucosinolate metabolism
216	glutathione S-transferase ERD13 (GSTF10)^Neg	gi\|15224582	5.82/33.0	5.54/29.8	66	5	2	0.11±0.00	0.17±0.00	1.51	0.00	0.17±0.10	0.09±0.09	-1.88	0.37
327	myrosinase-binding-like protein	gi\|30684083	5.14/76.0	5.12/48.5	131	21	6	0.05±0.01	0.04±0.00	-1.15	0.23	0.08±0.02	0.04±0.01	-2.10	0.02
509	sulfotransferase 18	gi\|15221130	5.66/57.0	5.52/40.7	177	13	4	0.01±0.00	0.00±0.00	-9999	0.01	0.00±0.00	0.00±0.00	—	—
523	3-isopropylmalate dehydrogenase^up	gi\|15241338	5.04/58.0	5.75/44.2	88	2	1	0.08±0.00	0.05±0.01	-1.63	0.00	0.00±0.00	0.00±0.00	—	—
556	amidase 1	gi\|18390964	6.08/61.0	5.83/45.4	152	22	6	0.02±0.01	0.00±0.00	-9999	0.04	0.02±0.03	0.00±0.00	-9999	0.19
864	thioglucoside glucohydrolase 1 (TGG1)	gi\|15809938	5.24/121.0	5.67/61.7	141	10	4	0.02±0.01	0.00±0.00	-9999	0.02	0.00±0.00	0.00±0.00	—	—
	Stress and defense
113	glutathione S-transferase DHAR1^Pos	gi\|8778432	5.66/31.9	6.82/50.6	150	6	2	0.12±0.03	0.07±0.02	-1.75	0.05	0.48±0.42	0.31±0.02	-1.56	0.00
161	putative glyoxalase	gi\|9828630	5.08/38.9	6.97/40.3	85	12	3	0.04±0.02	0.00±0.00	-9999	0.02	0.05±0.01	0.02±0.01	-2.22	0.01
183	glutathione S-transferase (AtGSTF3)	gi\|497788	6.40/29.1	5.80/23.5	116	11	2	0.03±0.01	0.00±0.00	-9999	0.00	0.08±0.00	0.01±0.00	-14.16	0.00
203	putative arginase^Pos	gi\|15236635	6.01/44.0	5.90/38.1	163	12	4	0.11±0.01	0.13±0.01	1.14	0.05	0.30±0.02	0.19±0.03	-1.61	0.01
275	HSP20-like chaperones protein	gi\|3193303	4.17/36.0	4.42/28.0	103	16	4	0.04±0.01	0.00±0.00	-9999	0.00	0.02±0.01	0.00±0.00	-9999	0.09
338	putative plastid-lipid-associated protein 1	gi\|15233357	4.52/41.0	5.45/35.0	161	23	5	0.02±0.01	0.00±0.00	-9999	0.00	0.13±0.08	0.03±0.03	-4.55	0.09
408	O-acetylserine (thiol) lyase B (oasB)	gi\|15224351	5.59/46.0	8.13/42.0	183	29	8	0.02±0.00	0.10±0.01	4.70	0.00	0.02±0.01	0.00±0.00	-9999	0.12
437	O-acetylserine (thiol) lyase isoform C (oasC)	gi\|6899947	5.75/49.0	6.96/41.5	96	12	3	0.02±0.01	0.00±0.00	-9999	0.01	0.00±0.00	0.00±0.00	—	—
872	luminal binding protein (BiP2)	gi\|1303695	5.16/122.0	5.08/73.7	165	11	6	0.04±0.01	0.03±0.00	-1.67	0.04	0.00±0.00	0.00±0.00	—	—
	Photosynthesis
126	chlorophyll a/b-binding protein	gi\|13265501	5.47/23.9	6.52/26.1	178	9	3	0.12±0.01	0.19±0.01	1.60	0.00	0.07±0.00	0.06±0.00	-1.08	0.13
170	chloroplast NAD-MDH	gi\|3256066	6.18/39.8	8.48/42.6	295	14	5	0.17±0.11	0.03±0.03	-5.88	0.10	0.13±0.01	0.09±0.02	-1.55	0.03
233	coproporphyrinogen III oxidase	gi\|240254000	5.51/50.0	6.24/44.1	155	35	9	0.07±0.00	0.07±0.00	-1.02	0.14	0.10±0.01	0.06±0.00	-1.62	0.01
247	uroporphyrinogen decarboxylase 2	gi\|15226690	6.72/54.0	8.29/43.7	517	32	11	0.07±0.00	0.07±0.00	-1.05	0.10	0.03±0.01	0.00±0.00	-9999	0.01
346	ribulose bisphosphate carboxylase	gi\|1944432	5.93/81.0	6.12/48.0	167	16	7	1.04±0.68	0.13±0.09	-8.33	0.08	1.00±0.26	0.00±0.00	-9999	0.00
865	transketolase-like protein	gi\|7329685	5.56/120.0	5.80/81.9	113	17	8	0.08±0.00	0.15±0.02	1.84	0.01	0.00±0.00	0.00±0.00	—	—
	Carbohydrate metabolism
272	3-oxoacyl-[acyl-carrier protein] reductase (NADH)	gi\|15229203	6.40/36.1	6.12/28.2	61	9	2	0.03±0.01	0.00±0.00	-9999	0.00	0.03±0.00	0.03±0.00	-1.31	0.05
766	2-oxoglutarate dehydrogenase, E3 subunit	gi\|4210334	6.40/95.0	6.96/54.0	137	34	5	0.02±0.00	0.00±0.00	-9999	0.00	0.00±0.00	0.00±0.00	—	—
	Protein synthesis, folding and destination
38	40S ribosomal protein S12-1	gi\|15218373	5.05/20.0	5.38/15.7	73	22	2	0.08±0.01	0.02±0.00	-3.76	0.00	0.08±0.01	0.03±0.01	-2.37	0.01
178	ATP-dependent Clp protease proteolytic subunit 2	gi\|18420643	6.40/29.1	6.71/26.4	92	14	3	0.02±0.01	0.00±0.00	-9999	0.01	0.00±0.00	0.00±0.00	—	—
799	TCP-1/cpn60 chaperonin family protein	gi\|15231255	5.19/101.0	5.60/63.7	308	27	12	0.01±0.01	0.00±0.00	-9999	0.02	0.00±0.00	0.00±0.00	—	—
	other
30	SRPBCC ligand-binding domain-containing protein	gi\|15236566	6.41/18.1	5.91/17.6	72	15	2	0.12±0.08	0.11±0.06	-1.08	0.89	0.34±0.03	0.00±0.00	-9999	0.00

^a Assigned spot number as indicated in Figure 1. ^b Database accession numbers according to NCBInr. ^c Experimental mass (kDa) and pI of identified proteins. ^d Theoretical mass (kDa) and pI of identified proteins.^e Mascot score reported after searching against the NCBInr database. ^f Sequence coverage. ^g Number of peptides sequenced. ^h Spot abundance change is expressed as the ratio of intensities (vol.%) of up-regulated (positive value) or down-regulated (negative value) proteins between MeJA-treated jin1-9 and WT. ^Neg Negatively regulated by MYC2. ^Pos Positively regulated by MYC2. ^up Up-regulated by MeJA. Paired Student's t-test was used to define the significant changes ( p <0.05). -9999 indicates the protein spots were not detectable in jin1-9 after MeJA treatment, short string indicates the protein spots were not detectable in WT and jin1-9 after MeJA treatment. Three treatments were performed.

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ISSN: 1477-5956

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