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Table 1 Significantly up-regulated proteins in the CD133 + Huh7 cells

From: Label-free quantitative proteomics of CD133-positive liver cancer stem cells

Accession

Protein description

Score

Unique peptides

Hepatocyte area

CD133 - Huh7 area

CD133 + Huh7 area

IDEAL-Q CD133 + /CD133 -

IDEAL-Q CD133 + /Hepatocyte

P-value a

MaxQuant CD133 + /CD133 -b

Plasma membrane c

IPI00216138

Transgelin

552

10

0.21 ± 0.05

0.13 ± 0.06

2.11 ± 0.30

15.96

9.99

<0.001

33.94

 

IPI00012540

Prominin-1 (CD133)

170

4

0.01 ± 0.01

0.05 ± 0.01

0.69 ± 0.09

15.24

76.22

<0.001

124.06

O

IPI00418471

Vimentin

1726

30

0.38 ± 0.03

0.82 ± 0.06

6.77 ± 0.47

8.24

17.77

<0.001

21.51

O

IPI00218918

Annexin A1

303

5

0.02 ± 0.002

0.04 ± 0.01

0.33 ± 0.03

7.83

21.93

<0.001

101.44

O

IPI00186460

Collagen alpha-1(II) chain

227

4

0.01 ± 0.01

0.15 ± 0.05

0.94 ± 0.06

6.10

67.07

<0.001

11.95

 

IPI00024095

Annexin A3

919

15

0.15 ± 0.003

0.44 ± 0.07

2.69 ± 0.48

6.10

17.70

<0.001

17.13

O

IPI00329650

Nucleoporin NUP53

252

4

0.05 ± 0.02

0.28 ± 0.04

1.29 ± 0.13

4.71

26.41

<0.001

3.62

O

IPI00296215

Tumor-associated calcium signal transducer 1 (EpCAM)

204

3

0.01 ± 0.004

0.12 ± 0.01

0.31 ± 0.02

2.56

27.73

0.010

6.17

O

IPI00386208

Gastric-associated differentially-expressed protein YA61P

224

4

0.01 ± 0.01

0.42 ± 0.04

0.97 ± 0.07

2.34

108.22

0.008

13.90

 

IPI00022977

Creatine kinase B-type

1181

19

0.86 ± 0.15

6.39 ± 0.58

13.50 ± 2.43

2.11

15.66

0.009

2.03

 
  1. a Significance B based on log(2) transformed ratios and intensity between CD133+ Huh7 cells and CD133- Huh7 cells.
  2. b The ratio was calculated by MaxQuant label-free quantification.
  3. c Proteins possibly locate on plasma membrane from Gene Ontology Cellular Component (GOCC) database.