Figure 3From: MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration MALDI-TOF (A) and HPLC-UV (B) analysis of tryptic digests of BSA in water, 20% AcN, and 6 M guanidine HCl. The inverted spectra in panel A represent digests with trypsin compared to an appropriate solvent control. HPLC-UV chromatograms in panel B show peptide peaks after trypsin digestion in water and 20% AcN and the parent peak for undigested BSA protein in the presence of 6 M guanidine HCl. Each sample was prepared in triplicate and one representative spectrum or chromatogram is presentedBack to article page