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Table 1 Optimized transitions selected for the quantification of FIBA, CO3, and CO4 peptides and their heavy analogues

From: MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration

Protein name

Peptide sequence

Form

Precursor m/z

Precursor charge

Product m/z

Product charge

Fragmention

DP

CE

FIBA

DSGEGDFLAEGGGVR

Light

733.33

2

758.42

1

y8

85

38.3

   

733.33

2

645.33

1

y7

85

38.3

   

733.33

2

445.25

1

y5

85

38.3

  

Heavy

735.84

2

761.43

1

y8

85

38.3

   

735.84

2

648.34

1

y7

85

38.3

   

735.84

2

448.26

1

y5

85

38.3

CO3

SSKITHRIHWESASLL

Light

622.34

3

810.92

2

b14

80

27.9

   

622.34

3

867.46

2

b15

80

26.4

  

Heavy

624.68

3

810.92

2

b14

80

27.9

   

624.68

3

870.97

2

b15

80

26.4

  

Light

467.01

4

767.40

2

b13

70

21.7

   

467.01

4

810.92

2

b14

70

17.2

  

Heavy

468.76

4

767.40

2

b13

70

21.7

   

468.76

4

810.92

2

b14

70

17.2

CO4

NGFKSHALQLNNRQI

Light

580.65

3

885.49

1

y7

85

29.6

   

580.65

3

757.43

1

y6

85

31.1

   

580.65

3

644.35

1

y5

85

31.1

  

Heavy

582.99

3

892.51

1

y7

85

29.6

   

582.99

3

764.45

1

y6

85

31.1

   

582.99

3

644.35

1

y5

85

31.1

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