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Table 1 Relative levels of potato leaf protein spots exhibiting a more than twofold decrease or increase for at least one stress treatment (see Figure 2 for protein spot numbering) a

From: Wounding, insect chewing and phloem sap feeding differentially alter the leaf proteome of potato, Solanum tuberosum L.

  

Stress b

  

Spot

Protein (NCBI Accession No.) c

Wounding

Beetles

Aphids

14

 

1.3

0

4.6

15

EST537399 similar to a subtilase (NP_199378)

1.6

0

4.1

72

Rubisco subunit binding-protein alpha subunit (P08824)

1.9

4.1

1.0

76

EST396117 similar to protein disulfide isomerase (Q9XF61)

0.6

3.2

1.0

81

 

0.4

8.5

0.8

104

ATP synthase β subunit (AAM52206)

1.3

0.4

3.5

114

 

0.5

0

1.0

124

 

1.1

2.5

1.0

172 d

Rubisco activase (AAC15236)

0.7

0.9

1.3

198

 

1.1

0.3

0.8

204

 

0.8

0

0.6

230

 

0.8

0.3

1.3

260

 

0.9

0

0.8

298 d

33-kDa PSII oxygen evolving complex protein (CAA35601)

0.9

0.9

1.0

301

 

1.3

0.4

0.9

304

Rubisco large subunit fragment (CAA70392)

0.9

0

1.0

306

 

1.2

0

1.5

328

 

2.5

2.7

0.9

346

 

2.5

2.3

0.7

349

Putative l-ascorbate peroxidase, chloroplastic (Q9THX6)

1.1

0.2

1.4

396

Cysteine protease inhibitor 7 (O24385)

1.7

2.6

0.8

421 d

23-kDa PSII oxygen evolving protein (CAA67696)

0.9

1.0

1.1

426

Aspartic protein inhibitor 3 (P58518)

2.0

0.6

1.0

444

Photosystem I reaction center subunit II (P12372)

2.5

0

1.2

445

 

2.3

0

2.4

450

 

1.8

0

2.4

451

Photosystem I reaction center subunit IV B (Q41229)

1.5

0

2.4

461

Photosystem I reaction center subunit IV (P12354)

1.3

0

4.3

464

Aspartic protease inhibitor (CAA45723)

1.3

2.9

1.4

469

Aspartic protein inhibitor 3 (P58518)

1.0

0

1.0

504

Pathogenesis-related protein P2

1.5

1.3

4.1

526

  

N (0.029)

 

527

 

N (0.011)

N (0.056)

 

528

  

N (0.089)

 
  1. aStatistical significance of the observed variations was confirmed by a one-way analysis of variance, using a significance threshold (α value) of 0.05.
  2. bData refer to average normalized volumes of the spots, divided by the average normalized volume for the corresponding spot in control gels. N stands for proteins induced de novo following stress treatment. Numbers in parentheses indicate averaged normalized volumes.
  3. cInformation on MS data, protein identifications and 2-DE coordinates is given in Additional files 1 and 2.
  4. dUnmodulated, negative controls found at comparable levels in treated and control plants.