Figure 1

S-nitrosylation of parkin, β-arrestin 2, and GAPDH in neuroblastoma cells treated with MPP⁺ . Parkin (A) or β-arrestin 2 (B) were overexpressed in SH-SY5Y cells. The SH-SY5Y cells were then treated with MPP⁺ (100 μM) for 3 h, and lysates prepared from the treated cells were analyzed by the SNO-RAC assay, in which ascorbate-dependent capture of proteins by resin indicates the presence of S-nitrosylated cysteine residues. Elutants were subjected to immunoblot analysis using anti-FLAG (A, B) or anti-GAPDH (C) antibody.