Figure 4

Illustration of the procedure to synthesize nonsense protein sequence from genomic sequences with confirmed exon positions. A genomic sequence with known exon positions (black, dark grey) is read and translated in three different ways, with different starting codon positions. Parts of an obtained amino acid sequence that align perfectly to parts of the confirmed protein are discarded (dark grey), while the remainder is kept as a synthesized nonsense sequence (light grey). The schema is simplified, true sequences and exons are longer than the ones depicted here.