Figure 1

A schematic overview of the whole-gel procedure (WG) vs. the in-gel digestion procedure (IGD). A. The number of pipettes next to the workflow indicates the amount of manual processing. B. manual processing steps, incubation and gel slicing time breakdown for day 1 for WG and IGD procedures for 1 sample (10 bands) and 9 samples (90 bands). C manual processing steps and incubation time breakdown for day 2. Details for Figure 1B and 1C can be found in Additional file 1: Table 1. D. Gel image showing the location of gel slices IGD 1–5 processed by in-gel digestion (empty regions) and the corresponding counterpart WG 1–5 region processed by the whole-gel procedure for HCT116 cell lysate.