Table 1 Number of protein identifications using the whole gel (WG) procedure vs in gel digestion (IGD)
From: Whole gel processing procedure for GeLC-MS/MS based proteomics
Human HCT116 cell lysate | Mouse tumor tissue lysate | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Gel Band | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | 6 |
Total ID IGD | 178 | 252 | 300 | 301 | 93 | 86 | 113 | 70 | 100 | 28 | 23 |
Total ID WG | 178 | 257 | 306 | 315 | 92 | 56 | 115 | 78 | 114 | 52 | 62 |
Total ID | 192 | 262 | 314 | 324 | 95 | 86 | 121 | 81 | 114 | 52 | 62 |
Overlap ID | 164 | 247 | 292 | 292 | 90 | 56 | 107 | 67 | 100 | 28 | 23 |
Only IGD | 14 | 5 | 8 | 9 | 3 | 30 | 6 | 3 | 0 | 0 | 0 |
Only WG | 14 | 10 | 14 | 23 | 2 | 0 | 8 | 11 | 14 | 24 | 39 |
Overlap* | 85% | 94% | 93% | 90% | 95% | 65% | 88% | 83% | 88% | 54% | 37% |
Median Mr (kDa) | 176 | 83 | 44 | 23 | 12 | 124 | 69 | 38 | 29.5 | 19 | 15 |