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Figure 2 | Proteome Science

Figure 2

From: Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells

Figure 2

Proliferating and differentiating NestinGFP+ NSCs isolated from E12-E15.5 embryonic forebrains differentially express HMGB2. (A) qRT-PCR analysis of HMGB2 mRNA expression in proliferating forebrain NSCs isolated at different time points during neural development. (B) qRT-PCR analysis of HMGB2 mRNA expression in differentiating forebrain NSCs isolated at E12, E14.5, and E15.5 and compared to age-matched proliferating NSCs. Note, in differentiating NSC analysis proliferating NSCs were set to 1. (C) HMGB2 protein is expressed in proliferating E12-E17.5 NSCs and is differentially expressed in differentiating E12 NSCs. Quantitative western blots of HMGB2 in proliferating NSCs from embryonic day E12 to E17.5. (D) Quantitative western blot of HMGB2 in differentiating NSCs at 24 and 48 hours after initiation of differentiation. All values are mean+/- SEM, *p≤0.05, **p≤0.005. (E) In vivo expression pattern of HMGB2 at E15.5 in the VZ/SVZ and cortical plate.

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Proteome Science

ISSN: 1477-5956