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Figure 3 | Proteome Science

Figure 3

From: Members of the high mobility group B protein family are dynamically expressed in embryonic neural stem cells

Figure 3

Proliferating and differentiating NestinGFP+ NSCs isolated from E12-E15.5 embryonic forebrains differentially express HMGB family members. (A) qRT-PCR analysis of HMGB1, HMGB3, and HMGB4 mRNA expression in proliferating NSCs isolated at different time points during neural development. (B) qRT-PCR analysis of HMGB1, HMGB3, and HMGB4 mRNA expression in differentiating forebrain NSCs isolated at E12, E14.5, and E15.5 and compared to age-matched proliferating NSCs. Note, in differentiating NSC analysis proliferating NSCs were set to 1. (C) HMGB1, 3, and 4 proteins are expressed in proliferating E12-E17.5 NSCs, and B1 and B2 proteins are differentially expressed in differentiating E12 NSCs. Quantitative immunoblots of HMGB1, HMGB3, and HMGB4 in proliferating NSCs from embryonic day E12 to E17.5. (D) Quantitative western blot of HMGB1 in differentiating NSCs at 24 and 48 hours after initiation of differentiation. All error bars are mean+/- SEM, *p≤0.05, **p≤0.005.

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Proteome Science

ISSN: 1477-5956