A SELDI mass spectrometry study of experimental autoimmune encephalomyelitis: sample preparation, reproducibility, and differential protein expression patterns

Table 2 Reproducibility of homogenization and subcellular fractionation protocols

The coefficient of variation (CV%) for fourteen protein peaks from an ion exchange fraction (Q1) of the cytosolic fraction of pooled mouse brain tissue obtained for two homogenization protocols in which tissue was either thinly sliced prior to homogenization with a Brinkmann rotor homogenizer (method M1) or tissue was cut into ~250 mg blocks and homogenized with a Teflon pestle (method M2). The overall CV% for the peak intensities was 17.0% for method M1 and 27% for method M2.

ISSN: 1477-5956