A SELDI mass spectrometry study of experimental autoimmune encephalomyelitis: sample preparation, reproducibility, and differential protein expression patterns

Table 4 Protein identification

Fraction (Q)	Mass (m/z)	p-Value	Accession number	Entry name	Theoretical MW	MASCOT score	Peptides detected	Sequence coverage
2	6550	0.05	IPI00138892	Uba52	14719	642	7	46
			IPI00225390	COX6b1	10065	84	2	24
	10274	0.05	IPI00225390	COX6b1	10065	545	7	67
			IPI00120045	Hspe1-rs1	10971	431	3	33
			IPI00403381	Ndufv3	11806	430	4	36
	11691	0.05	IPI00109966	B2m	13814	499	4	27
			IPI00128345	Ndufs6	13012	414	3	31
	16836	0.05	IPI00890117	CfI1	18548	663	12	70
			IPI00229008	Ndufs4	19772	335	8	48
3	12448	0.05	IPI00115240	MBP1	27151	443	5	15
	13954	0.05	IPI00223381	MBP8	14202	584	12	61
4	9841	0.03	IPI00131771	COX6C	8464	1460	9	63
			IPI00223593	MBP10	20801	1049	11	50
			IPI00111770	Atp5k	8230	473	7	68
			IPI00225390	COX6b1	10065	443	6	53
			IPI00315302	Ndufa2	10909	428	5	41
5	16910	0.02	IPI00223379	MBP6	17215	2521	14	64
			IPI00123817	Atp6v1g2	13643	501	4	39
	17307	0.02	IPI00223380	MBP7	17230	1168	12	70
			IPI00319231	Rps13	17212	362	7	45
			IPI00229008	Ndufs4	19772	354	8	34
			IPI00131186	Btf3	17688	329	3	46

Proteins identified in 1D polyacrylamide gel bands which correspond to the molecular weights of differentially expressed proteins in EAE relative to controls found by SELDI-MS analysis. Only the myelin basic protein isoforms found in fraction Q3 are unambiguously identified.

ISSN: 1477-5956