Figure 1

Venn diagram analysis of MudPIT data sets. A, Categorization of proteins identified in Embryo experiment 1 and 2. The complete PINCH-PrA data set includes a subset of proteins identified as ‘PINCH-specific pull-down’. The proteins identified in wild type embryos are pulled down non-specifically by purification matrix. A complete list of these proteins is available in Additional file 1: Table S1. B, The union of both Embryo experiment data sets constitutes an inclusive list of candidate PINCH binding partners. The intersection of the two data sets (dark shaded) represents candidates replicated in both experiments. C, Composition of proteins identified in Drosophila S2 cell experiments. A complete list of these proteins is available in Additional file 2: Table S2. D, In the intersection of the S2 cell data set and the replicated proteins of the embryo data sets, 4 proteins were identified (indicated by * in Table 2). In the intersection of the S2 cell data set and the inclusive list of both embryo experiment data sets, 30 proteins were identified. The identities of these proteins are listed in Table 2.