Interactions by 2D Gel Electrophoresis Overlap (iGEO): a novel high fidelity approach to identify constituents of protein complexes

Table 2 Proteins identified in two or more MudPIT analyses

			Peptide coverage
Flybase ID	Gene symbol	Name	Embryo E1	Embryo E2	S2 cell
CG10504	Ilk	Integrin linked kinase (ILK)	36.6%	20.5%	35.7%	*
CG10687	Aats-asn	Asparaginyl-tRNA synthetase	14.2%		6.5%
CG10811	eIF4G	eukaryotic translation initiation factor 4G	2.8%		4.9%
CG10938	Prosα5	Proteasome α5 subunit	18.0%		22.5%
CG1100	Rpn5	Rpn5	9.0%		2.0%
CG11271	RpS12	Ribosomal protein S12	51.1%		17.3%
CG11522	RpL6	Ribosomal protein L6		6.6%	8.6%
CG12030	Gale	UDP-galactose 4'-epimerase	8.9%		22.0%
CG12233	l(1)G0156	lethal (1) G0156	19.5%		13.3%
CG1404	ran	ran		10.6%	14.4%
CG14207	CG14207		32.8%		24.0%
CG18290	Act87E	Actin 87E	32.4%		26.3%
CG18572	r	rudimentary	7.4%		3.7%
CG2512	αTub84D	α-Tubulin at 84D	42.4%		30.2%
CG31794	Pax	Paxillin	10.7%		11.5%
CG3186	eIF-5A	eIF-5A	28.3%		14.5%
CG32528	parvin	parvin	55.6%	30.2%	55.9%	*
CG32920	Prx5	Peroxiredoxin 5		18.4%	19.5%
CG3416	Mov34	Mov34	15.7%		18.3%
CG4046	RpS16	Ribosomal protein S16		12.8%	17.6%
CG4183	Hsp26	Heat shock protein 26	38.9%		39.4%
CG5119	pAbp	polyA-binding protein		6.5%	9.3%
CG5289	Pros26.4	Proteasome 26S subunit subunit 4 ATPase		8.2%	11.2%
CG6050	EfTuM	Elongation factor Tu mitochondrial	15.5%		8.6%
CG7954	stck	steamer duck (PINCH)	48.7%	29.3%	66.6%	*
CG8882	Trip1	Trip1	16.3%		14.7%
CG9031	ics	icarus (RSU-1)	21.7%	17.6%	63.2%	*
CG9412	rin	rasputin		1.2%	4.4%
CG9674	CG9674		1.8%		1.9%

Proteins with an asterisk (*) in the right column were identified in all three data sets, indicating they are bona fide constituents this protein complex.

ISSN: 1477-5956