Interactions by 2D Gel Electrophoresis Overlap (iGEO): a novel high fidelity approach to identify constituents of protein complexes

Table 3 Proteins of interest identified by iGEO and nano-LC-MS/MS analysis, cross-referenced to their presence in the MudPIT data sets

						Presence in MudPIT data set
Spot ID	Flybase ID	Protein ID	Function	MW	pI	Embryo 1	Embryo 2	S2 cell
38	CG16916	Rpt3	26S Proteasome Subunit	47	5.0	-	-	+
40	CG8983	ERp60	Protein disulfide isomerase homolog	55	5.6	-	-	+
63	CG1341	Rpt1	26S Proteasome Subunit	48	5.8	-	-	-
64	CG32528	Parvin	Parvin	42	6.6	+	+	+
66	CG8863	Droj2	DnaJ-like-2 chaperone	45	6.4	-	-	+
92	CG4904	Pros35	Proteasome 35kDa subunit	31	6.5	-	-	+
111	CG9031	RSU-1	RSU-1 isoform	31	6.6	+	+	+

Raw data of MASCOT search results are available in Additional file 7: MASCOT raw data.

ISSN: 1477-5956