Figure 3

Two-dimensional proteomic map of soluble proteins in E. coli DH5α. E. coli DH5α were treated in the absence of any distamycin A derivatives at 37°C for 10 hours, followed by cell protein solubilization via high intensity ultrasonication in rehydration buffer. The soluble protein (240 μg) was separated on an immobilized pH gradient gel strip (pH 4-7) and 12% SDS-PAGE gel. Proteins were visualized by staining with commercial SimplyBlue SafeStain. The numbers correspond to the numbers listed in Figure 4 and Table 1.