Figure 4

The expression of the proteins identified in E. coli DH5α after treatment with distamycin-A derivatives. E. coli DH5α were treated in the absence (control) or presence of 300 μM PySSPy, 300 μM PyRSPy or 300 μM 3A at 37°C for 10 hours, followed by cell protein solubilization and protein separation using an immobilized pH gradient gel strip (pH 4-7) and 12% SDS-PAGE gel. The gels were visualized using commercial SimplyBlue SafeStain. The resulting proteomic maps were shown in enlarged sections for comparison with the non-treated control. The numbers represent the proteins that show either up- or down-regulation in E. coli DH5α in response to PySSPy, PyRSPy and 3A, compared with the non-treated control. These numbers correspond to the numbers listed in Figure 3 and Table 1.