Table 1 Identification of differentially expressed proteins of E coli DH5α treated with PySSPy, PyRSPy and 3A
No. | ICBI accession No. | Protein identified | Calculated pI/MW (kDa) | Matched (Measured) peptide | Sequence coverage (%) | Relative intensities a | ||
|---|---|---|---|---|---|---|---|---|
PySSPy | PyRSPy | 3A | ||||||
1 | GLYA_EC024 | Serine hydroxymethyl-transferase | 6.03/94 | 9 (22) | 29 | -25 ± 04 | -2.1 ± 0.2 | -1.4 ± 0.1 |
2 | IR3Z2_ECOUT | Glycerol kinase | 5.97/60 | 15 (30) | 33 | -2.3±0.4 | -1.6±0.05 | -1.7±0.1 |
3 | DHSB_ECQU | Succinate dehydrogenase iron-sulfur subunit | 6.327 | 10 (21) | 47 | -2.4 ± 0.1 | -3.5 ± 0.3 | -3.4 ± 0.1 |
4 | KDUD_ECOLI | 2-dehydro-3-deoxy-D-gluconate 5-dehyrixenase | 5.24/27 | 8 (18) | 40 | +1.7 ± 0.1 | +2.0 ± 0.2 | +1.9 ± 0.2 |
5 | IvEH_ECO24 | Malate dehydrogenase | 5.61/32 | 11 (22) | 49 | +2.3 ± 0.2 | +3.1 ± 0.05 | +1.9 ± 0.06 |
6 | CH6O1_ECOK1 | 60 kDa chaperonin 1 | 4.85157 | 10 (26) | 33 | +2.4 ± 0.1 | +2.5 ± 0.2 | +3.1 ± 0.4 |
7 | MAO2_ECOLI | NADP-dependent malic enzyme | 5,3494 | 11 (13) | 14 | -21 ± 0.5 | -2.1±0.3 | NS |
8 | DPS_EC024 | DNA protection during starvation protein | 5.72/19 | 9 (1) | 58 | -2.3 ± 0.1 | -2.8 ± 0.6 | NS |
9 | PCKA_EC024 | Phosphoenolpyruvate carboxykinase | 5.46/60 | 14 (27) | 35 | -24 ± 0.2 | NS | NS |
10 | GCST_EC024 | Aminomethyltransferase | 5.36/40 | 9 (17) | 35 | -2.1 ± 0.3 | NS | NS |
11 | SODF_ECO57 | superoxide dismutase [Fe] | 5.58/53 | 15 (16) | 37 | NS | +1.9 ± 0.009 | NS |
12 | ATPB_EC024 | ATP synthase subunit beta | 4.9150 | 16 (50) | 50 | +3.0±0.2 | NS | +2.6 ± 0.2 |
13 | EFTIJ1_EC024 | Elongation factor Tu 1 | 53143 | 17 (44) | 53 | +3.3 ± 0.2 | NS | +3.1 ± 0.1 |
14 | TNAA_EC024 | Tryptophanase | 5.88/53 | 15 (16) | 37 | NS | +1.9 ± 0.009 | NS |
15 | PUR9_ECO5S | Bifunctional purine biosynthesis protein purH | 5.53/58 | 11 (14) | 31 | NS | +1.3 ± 0.2 | +1.6 ± 0.06 |
16 | DNAK_ECO24 | Chaperone protein dnaK | 4.83/69 | 16 (28) | 28 | NS | +3.0 ± 0.1 | +2.1 ± 0.2 |
17 | AHPC_ECO57 | Alkyl hydroperoxide reductase subunit C | 5.0/21 | 6 (11) | 43 | NS | +2.6 ± 0.3 | +2.5 ± 0.4 |
18 | ALKH_ECO57 | KHG/KDPG aldolase | 5.57/22 | 7 (20) | 46 | NS | +1.8 ± 0.2 | +2.1 ± 0.3 |