Figure 1

Changes in the protein profile of mouse hearts due to aging. Representative dual channel overlay of mice hearts 1 month old and 4 months old in the pH range of 4–7. A total of 1683 spots were detected on the gel using Delta 2D software (Decodon). Statistical analysis with Genespring (Agilent) revealed 176 spots with significantly altered intensities (p ≤ 0.05) of which 86 spots were identified by mass spectrometry. 40 (58) spots labeled in red showed ≥ 1.5 fold higher intensities, whereas 46 (118) spots labeled in green displayed > 1.5 fold lower intensities in the 4 months old mouse hearts compared to 1 month controls. Proteins which did not change in intensity appear in yellow.