Table 1 Proteins identified from the differential 2D DIGE analysis after TGEV infection
Spot number a | Protein name (Abbr.) | Accession number b | Molecular mass (kDa) | pI | Peptide matched c | Sequence coverage (%) | Protein score d | (TGEV/mock) | |
|---|---|---|---|---|---|---|---|---|---|
Average volume ratio e | p value e | ||||||||
Cytoskeleton proteins | |||||||||
Microfilament-associated proteins | |||||||||
1154 | beta actin (ACTB) | gi|45269029 | 44.76 | 5.55 | 3 | 36 | 285 | 3.64 | 0.000035 |
1308 | beta actin (ACTB) | gi|45269029 | 44.76 | 5.55 | 3 | 16 | 407 | 4.04 | 0.00013 |
1312 | beta actin (ACTB) | gi|45269029 | 44.76 | 5.55 | 3 | 14 | 129 | −2.82 | 0.00076 |
1368 | beta actin (ACTB) | gi|45269029 | 44.76 | 5.55 | 1 | 16 | 82 | 4.68 | 0.0097 |
1383 | beta actin (ACTB) | gi|45269029 | 44.76 | 5.55 | 5 | 34 | 537 | 3.7 | 0.0019 |
1647 | beta actin (ACTB) | gi|150438831 | 44.76 | 5.55 | 3 | 21 | 242 | 7.03 | 0.005 |
1675 | alpha tropomyosin (TPM1) | gi|158931149 | 32.69 | 4.71 | 5 | 46 | 544 | −2.82 | 0.0027 |
1864 | beta actin (ACTB) | gi|476332 | 26.10 | 5.55 | 3 | 35 | 177 | 2.58 | 0.00023 |
Microtubule-associated proteins | |||||||||
911 | alpha Tubulin (TUBA1B) | gi|116256086 | 50.12 | 4.94 | 4 | 28 | 340 | 9.08 | 0.0029 |
964 | beta Tubulin (TUBB) | gi|75045190 | 49.64 | 4.78 | 7 | 31 | 448 | 2.28 | 0.00081 |
978 | alpha Tubulin (TUBA1B) | gi|116256086 | 50.12 | 4.94 | 3 | 32 | 338 | 3.38 | 0.000014 |
Intermediate filament proteins | |||||||||
1078 | vimentin (VIM) | gi|335296459 | 53.64 | 5.06 | 6 | 64 | 1020 | −3.26 | 0.0001 |
1266 | vimentin (VIM) | gi|335296459 | 53.64 | 5.06 | 6 | 60 | 888 | −4.28 | 0.00013 |
1892 | vimentin (VIM) | gi|335296459 | 53.64 | 5.06 | 2 | 35 | 389 | −2.82 | 0.00041 |
1947 | vimentin (VIM) | gi|335296459 | 53.64 | 5.05 | 4 | 35 | 582 | 4.84 | 0.000014 |
1968 | vimentin (VIM) | gi|21431723 | 30.97 | 4.67 | 4 | 25 | 366 | 3.06 | 0.00036 |
1970 | vimentin (VIM) | gi|335296459 | 53.64 | 5.06 | 4 | 35 | 603 | 2.43 | 0.00082 |
1907 | keratin 19 (KRT19) | gi|311267276 | 44.19 | 5.05 | 8 | 47 | 682 | −3.99 | 0.000023 |
2090 | vimentin (VIM) | gi|21431723 | 30.97 | 4.67 | 2 | 9 | 120 | 5.63 | 0.0095 |
2280 | vimentin (VIM) | gi|335296459 | 53.64 | 5.06 | 6 | 34 | 504 | 9.65 | 0.0031 |
RNA processing | |||||||||
1606 | heterogeneous nuclear ribonucleoprotein U (HNRNPU) | gi|335296158 | 104.85 | 5.85 | 3 | 7 | 277 | −3.26 | 0.011 |
1803 | thiopurine S-methyltransferase (TPMT) | gi|311259781 | 28.46 | 5.46 | 5 | 41 | 497 | 3.59 | 0.000099 |
2023 | thiopurine S-methyltransferase (TPMT) | gi|311259781 | 28.46 | 5.46 | 5 | 54 | 476 | 6.96 | 0.000008 |
Protein biosynthesis and modification | |||||||||
716 | protein phosphatase 2A (PP2A) | gi|510469 | 65.28 | 5.00 | 2 | 30 | 239 | 2.71 | 0.0051 |
1116 | eukaryotic initiation factor 4A-I (EIF4A1) | gi|154147660 | 46.13 | 5.32 | 7 | 44 | 586 | 3.87 | 0.00052 |
1158 | eukaryotic initiation factor 4A-I (EIF4A1) | gi|154147660 | 46.13 | 5.32 | 5 | 40 | 531 | 3.37 | 0.0011 |
1509 | acidic ribosomal protein P0 (RPLP0) | gi|182705234 | 34.34 | 5.71 | 4 | 50 | 483 | 2.38 | 0.02 |
Vesicle transport | |||||||||
1602 | annexin A8 (ANXA8) | gi|194042330 | 36.71 | 5.20 | 6 | 63 | 752 | −3.31 | 0.0063 |
1814 | annexin A4 (ANXA4) | gi|4033507 | 35.81 | 5.71 | 5 | 49 | 510 | −5.96 | 0.000008 |
Mitochondrion associated protein | |||||||||
906 | cytochrome b-c1 complex subunit 1 (UQCRC1) | gi|335299041 | 52.67 | 5.76 | 4 | 38 | 342 | 7.9 | 0.000023 |
1094 | prohibitin (PHB) | gi|335308255 | 28.88 | 5.74 | 6 | 72 | 688 | 3.04 | 0.0031 |
Signal transduction | |||||||||
1871 | 14-3-3 protein eta (YWHAH) | gi|194043292 | 28.19 | 4.81 | 4 | 30 | 282 | −2.52 | 0.00035 |
Metabolism | |||||||||
1640 | L-lactate dehydrogenase B chain (LDHB) | gi|164518958 | 36.42 | 6.73 | 2 | 22 | 126 | 5.74 | 0.0016 |