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Table 1 Differentially expressed proteins identified by MS or MS/MS

From: Proteomic analysis of the inhibitory effect of epigallocatechin gallate on lipid accumulation in human HepG2 cells

Spot no.

Protein name

Accession no.a

Theoretical mr/pI

MOWSE score

Sequence coverage (%)

Protein expression b

Biological function

A

B

C

402

Keratin 1

H6VRF8

66184/8.15

118

13

Cytoskeleton

1210

Lysosomal protective protein

P10619

34065/5.49

211

11

Glycometabolism

1217

14-3-3 protein gamma

P61981

28325/4.80

350

30

Signal transduction

1311

Ubiquitin thioesterase OTUB1

Q96FW1

26652/5.22

331

31

DNA repair

2307

Serine/arginine-rich splicing factor 7

Q16629

26224/11.77

161

15

mRNA processing

3111

Probable G-protein coupled receptor 179

Q6PRD1

260635/5.54

63

6

Signal transduction

3208

Prohibitin

Q6PUJ7

29859/5.57

154

23

Chaperone proteins

3214

C447E6.1 (Nucleotide binding protein 1)

Q2YS46

28604/5.89

65

16

Chaperone proteins

4116

Ferritin light chain

P02792

18818/5.65

158

36

Iron storage

4201

Phosphoserine phosphatase

P78330

25163/5.53

110

22

Protein phosphorylation

4411

Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial

P45954

44681/5.83

69

12

Lipid metabolism

5308

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

P62136

38229/5.94

239

29

Protein phosphorylation

5414

Galactokinase

P51570

42702/6.04

131

14

Glycometabolism

6205

Peroxiredoxin-6

P30041

25133/6.60

70

18

Antioxidant defense

6211

Platelet-activating factor acetylhydrolase IB subunit gamma

Q15102

25832/6.33

136

26

Lipid metabolism

6403

Septin-2

Q15019

41689/6.15

123

21

Cytoskeleton

6707

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

P31040

73672/7.06

170

25

Respiration

8012

Cofilin-1

P23528

18719/8.22

128

31

Signal transduction

  1. a SwissProt accession number.
  2. b A: control group. B: FFA group. C: FFA + EGCG (50 μM) group.