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Figure 4 | Proteome Science

Figure 4

From: A new type of protein chip to detect hepatocellular carcinoma-related autoimmune antibodies in the sera of hepatitis C virus-positive patients

Figure 4

Improvement of correlation between dual signals by SDS/2ME/heat pre-treatment. Alexa555 fluorescence is plotted vs. Alexa633 fluorescence for each spot for chips spotted with HSP70C and pre-treated with SDS/2ME/heat (closed circles and bold line) or not pre-treated (open circles and dotted line) before incubating with serum no. 193. Each graph contains sixty spots from 10 chips for four different experiments. R2 values were determined by linear regression analysis. Detection of HSP70C was enhanced by pre-treatment with SDS/2ME/heat.

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ISSN: 1477-5956