Table 1 List of up- or down-regulated proteins identified by LC-MS/MS due to the effect of age (A), time in transit (T), the interaction between age and time in transit (AxT), their function, the mean percentage volumes (%V), and the fold change ratios (FC), considering older vs. younger chickens (O/Y), longer vs. shorter transit times (L/S), and older birds in transit for longer (OL) vs. older birds with shorter transit times (OS)

34

A

0.045

O/Y-2.61

PDC6I_MOUSE

Programmed cell death 6-interacting protein

138

5

96520/6.16

Apoptosis, Cell cycle, Cell division, Protein transport, Transport

Q9WU78

104

A

0.017

3.29

ANXA6_CHICK

Annexin A6

462

13

75628/5.42

Calcium ion binding, calcium-dependent phospholipid binding

P51901

159

A

0.027

2.00

2AAA_HUMAN

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

57

2

66065/5.00

Chromosome partition

P30153

527

A

0.022

1.61

ANXA5_CHICK

Annexin A5

352

10

36290/5.60

Negative regulation of coagulation, Calcium ion binding

P17153

617

A

0.027

1.76

PGK_CHICK

Phosphoglycerate kinase

115

4

45087/8.31

ATP binding. Glycolysis

P51903

631

A

0.021

1.87

PRDX6_CHICK

Peroxiredoxin-6

113

4

25075/5.73

Peroxidase activity. Lipid degradation

Q5ZJF4

663

A

0.029

1.85

ENOB_CHICK

Beta-enolase

72

2

47566/7.27

Phosphopyruvate hydratase activity. Glycolysis

P07322

732

A

0.016

1.58

HSP7C_CHICK

Heat shock cognate 71 kDa protein

361

10

71424/-

Stress response. ATP binding, Chaperone

O73885

77

T

0.076

L/S 2.08

THOP1_BOVIN

Thimet oligopeptidase

66

2

79002/5.83

Hydrolase, Metalloprotease, Protease

Q1JPJ8

477

T

0.041

1.74

G3P_CHICK

Glyceraldehyde-3-phosphate dehydrogenase

75

2

35909/8.71

Glycolysis, Apoptosis, NAD or NADH binding

P00356

671

T

0.090

1.60

HSPB1_CHICK

Heat shock protein beta-1

647

7

21715/5.77

Actin capping. Stress response

Q00649

676

T

0.041

1.61

KPYK_CHICK

Pyruvate kinase muscle isozyme

146

3

58434/7.28

ATP, magnesium and potassium binding, Pyruvate kinase activity. Glycolysis

P00548

76

AxT

0.052

OL/OS 2.58

PYGM_MOUSE

Glycogen phosphorylase, muscle form

226

7

97681/6.65

Carbohydrate metabolism, glycogen metabolism

Q9WUB3

666

AxT

0.094

2.04

TPIS_CHICK

Triosephosphate isomerase

290

8

26832/6.79

Fatty acid biosynthesis, Gluconeogenesis, Glycolysis, Lipid synthesis, Pentose shunt

P00940