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Table 2 Volume intensity, measured as pixels per area considered, were calculated using the image lab 3.0 (Biorad) software, drawing boxes of equal area around the whole lanes of the Sypro Ruby stained membrane, or close to the blot bands

From: A note on protein expression changes in chicken breast muscle in response to time in transit before slaughtering

 

Volume intensity

Blot/SyproR%

 

Sypro ruby

PGYM

Enolase

PGYM

Enolase

YS

94,874,384

3,791,340

767,025

4.00

0.81

YL

107,562,330

3,738,332

679,206

3.48

0.63

OS

84,668,383

5,148,930

1,150,182

6.08

1.36

OL

79,410,020

9,381,180

1,200,996

11.81

1.51

  1. Values refer to Figure 2a for total protein quantification, and to Figures 2b and 2c for visualization with anti-human PYGM and anti-human enolase antibodies, respectively. The ratios of the blot band to total protein densitometric values are reported.
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