No.
1
| Ref.
2
| Name
3
| IPG 4-7
4
| IPG 3-10
5
| Nephge 3-10
6
|
|---|
Standard
5
| High load
5
| Standard
6
| High load
6
|
|---|
a | 1 | SSA1/2 | 2.4 ± 0.2 | 1,6 ± 0,1 | 1,6 ± 0,4 | 2,6 ± 0,3 | 2,0 ± 0,2 |
b | 2 | SSA1/2 |
c | 3 | SSA4 |
d | 4 | KAR2 | 3.8 ± 0.4 | 2,7 ± 0,5 | 1,8 ± 0,4 | 9,0 ± 3,1 | 2,5 ± 0,2 |
e | 5 | SSE1 | 2.3 ± 0.2 | 1,4 ± 0,3 | 1,8 ± 0,7 | 2,2 ± 0,8 | 1,7 ± 0,1 |
f | 6 | HSC82 | 2.1 ± 0.3 | 2,0 ± 0,2 | 2,1 ± 1,0 | - - | - - |
6 | HSP82 |
g | 7 | ENO2 | 1.5 ± 0.2 | 1,4 ± 0,3 | 1,1 ± 0,2 | 1,3 ± 0,2 | 1,1 ± 0,1 |
h | N.I.7
| SSA1/27
| 2.2 ± 0.3 | 1,5 ± 0,1 | 1,1 ± 0,1 | 1,6 ± 0,3 | 2,1 ± 0,4 |
?8
| N.A. | GPM1 | N.A. | 2,2 ± 1,3 | 0,7 ± 0,2 | 1,3 ± 0,3 | 1,0 ± 0,1 |
!8
| N.A. | SIS1 | N.A. | - - | - - | 2,6 ± 0,4 | 2,2 ± 0,2 |
-
1Differentially expressed protein spots in this experiment are indicated by letters (see Figures 1 and 2).
-
2The same protein spots are indicated by the numbers in the referenced article ([17], see Figure nine and Table one).
-
3Accepted name from the Saccharomyces genome database (SGD) and YPD. Spots 1 and 2 represent mixtures of similar proteins Ssa1 and Ssa2 (97% identity) at an unknown ratio (see Table one legend in [17]).
-
4Cellular protein expression fold change in MeH expressing versus control cells that was determined in previous work using pH4-7 IPG-based 2DE system (Invitrogen); the values are taken from the Table one in reference [17].
-
5Expression fold changes of the same proteins determined from independent experiments in this work using pH3-10 IPG strips (Invitrogen); ~50 μg of whole cell protein was loaded onto IPG strips in “Standard” experiment, whereas ~100 μg was used in a “High load” experiment.
-
6Expression fold changes of the same proteins determined from independent experiments in this work using pH3-10 NEPHGE first dimension gels (WITAvision); ~30 μg of whole cell protein was loaded onto NEPHGE gels in “Standard” experiment, whereas ~100 μg was used for each gel in the “High load” analysis.
-
7Not identified (N.I.) in previous study, because increased amount of this protein was observed only in cells expressing MeH, but not MuHN protein (the expression fold change in MeH/Control cells determined by IPG4-7 system here is given from our unpublished data).
-
8? and ! indicate basic protein spots (pI > 7) that were not analysed in previous experiment on pH4-7 platform (N.A. – not assayed). Despite that protein spot “?” showed false expression change (“artefact”) in IPG-based system (unreliable expression changes are apparent by high error range), in this experiment it was identified as phosphoglycerate mutase 1 (Gpm1p). Protein Sis1p in this study was identified using NEPHGE-based 2DE system, whereas it was not detected by IPG-based 2DE method.
