Figure 7

Validation of protein fragment by immunoblotting. (A) Levels of the UBA1 protein did not differ among the leukemia and normal cells. Isoform 1 of fibrinogen alpha chain precursor and PF4 immunoreactive bands show that weak or no bands are seen in newly diagnosed and refractory & relapsed AML cases. (B) Densitometry comparison of UBA1 protein relative to β-actin as determined by western blot analysis in figure A. (C) Densitometry comparison of isoform 1 of fibrinogen alpha chain precursor protein relative to β-actin as determined by western blot analysis in figure A. (D) Densitometry comparison of PF4 protein relative to β-actin as determined by western blot analysis in figure A. (UBA1: ubiquitin-like modifier activating enzyme 1; FGA: isoform 1 of fibrinogen alpha chain precursor; PF4: platelet factor 4; AML-CR: AML complete remission; AML-ND: AML newly diagnosed; AML-RR: AML refractory & relapsed).