The proteome of the calcified layer organic matrix of turkey (Meleagris gallopavo) eggshell

Mann, Karlheinz; Mann, Matthias

doi:10.1186/1477-5956-11-40

Proteome Science

Table 1 The most abundant proteins of turkey eggshell matrix

From: The proteome of the calcified layer organic matrix of turkey (Meleagris gallopavo) eggshell

ENSMGAP and UniProtKB	Probable chicken homolog	Sequence	FASTA	Protein	% of total¹
ENSMGAP and UniProtKB		identity	E-value
Accession		identity	E-value
00000007641	IPI00581368	79.8%	1.9^-135	Ovocleidin-116 **+	31.30
(G1N6E1)	OC116	79.8%	1.9^-135	Ovocleidin-116 **+	31.30
00000004038	IPI00583974	90.9%	2.6^-150	Ovalbumin **	12.52
(G1MYK6)	OVAL	90.9%	2.6^-150	Ovalbumin **	12.52
0000015961	IPI00595980	99.2%	0	Periostin	10.89
(G1NQ91)	F1P4N9	99.2%	0	Periostin	10.89
00000007759	IPI00573506	78.8%	8.8^-151	Ovocalyxin-36 **+	6.00
(G1N6M8)	Q53HW8	78.8%	8.8^-151	Ovocalyxin-36 **+	6.00
00000000142	IPI00600069	87.6%	1.3^-68	Extracellular fatty acid-binding protein (EXFAB) **	3.82
(G1MQ16)	EXFAB	87.6%	1.3^-68	Extracellular fatty acid-binding protein (EXFAB) **	3.82
0000018878	IPI00588727	77.9%	3.7^-60	EDIL3 **, fragment	3.70
(G3UT43)	F1NCN3	77.9%	3.7^-60	EDIL3 **, fragment	3.70
00000018172	IPI00685019	83.2%	5.2^-72	Ig light chains with overlapping peptide sets ** ²	2.81
(G3UR71)	F1NSD0
etc.	etc.
00000001491	IPI00601265	86.8%	5.0^-57	Avidin *	2.67
(G1MSZ9)	AVID	86.8%	5.0^-57	Avidin *	2.67
00000010561	IPI00574195	88.4%	0	Serum albumin **	2.57
(G1NCR2)	LBU	88.4%	0	Serum albumin **	2.57
00000002725	IPI00683271	87.3%	2.2^-203	Ovotransferrin **	2.22
(G1MVN4)	TRFE	87.3%	2.2^-203	Ovotransferrin **	2.22
00000007002	IPI00576782	90.6%	2.2^-56	Cystatin **	1.67
(G1N522)	CYT	90.6%	2.2^-56	Cystatin **	1.67
0000019196	IPI00578559	76.9%	4.0^-138	Polymeric Ig receptor **, fragment	1.59
(G3UTW1)	F1NE61	76.9%	4.0^-138	Polymeric Ig receptor **, fragment	1.59
00000008902	IPI00575013	95.7%	2.2^-191	Similar to milk fat globule EGF factor 8 protein **	1.25
(G1N944)	E1C0K5	95.7%	2.2^-191	Similar to milk fat globule EGF factor 8 protein **	1.25
0000015436	IPI00583503	97.6%	2.2^-58	Similar to trefoil family peptide 2 (TFF-2)	1.13
(G1NP11)	E1BZ37	97.6%	2.2^-58	Similar to trefoil family peptide 2 (TFF-2)	1.13
00000002528	IPI00571581	93.1%	2.7^-173	Sulfhydryl oxidase 1 **	1.08
(G1MV84)	QSOX1	93.1%	2.7^-173	Sulfhydryl oxidase 1 **	1.08
00000005746	Q90WR3	87.1%	1.2^-76	Hemopexin, fragment **	0.80
(H9H0W2)	Q90WR3	87.1%	1.2^-76	Hemopexin, fragment **	0.80
00000005136	IPI00680258	89.3%	2.1^-150	Similar to α2-antiplasmin/serpinF2 *+	0.80
(G1N0Z7)	F1NAR5	89.3%	2.1^-150	Similar to α2-antiplasmin/serpinF2 *+	0.80
00000005175	IPI00587267	97.9%	5.7^-173	Similar to serpinF1/PEDF/α2-antiplasmin **	0.64
(G1N131)	E1C7H6	97.9%	5.7^-173	Similar to serpinF1/PEDF/α2-antiplasmin **	0.64
00000010985	IPI00600859	95.2%	8.9^-70	Lysozyme C **	0.64
(LYSC)	B8YK79	95.2%	8.9^-70	Lysozyme C **	0.64
00000017273	IPI00599414	95.9%	1.4^-100	SPARC/BM-40/osteonectin *	0.57
(G3UNZ0)	SPRC	95.9%	1.4^-100	SPARC/BM-40/osteonectin *	0.57
00000004416	IPI00573738	88.9%	5.0^-120	Ovalbumin-reated protein Y *	0.43
(G1MZF4)	OVALY	88.9%	5.0^-120	Ovalbumin-reated protein Y *	0.43
00000013361	IPI00595705	98.4%	1.0^-54	Similar to epidymal secretory protein E1 *	0.43
(G1NJ71)	Q5ZJI7	98.4%	1.0^-54	Similar to epidymal secretory protein E1 *	0.43
00000016597	IPI00822809	77.8%	7.5^-22	Meleagrin (gallin)	0.42
(G1NRF9)	DGGR58	77.8%	7.5^-22	Meleagrin (gallin)	0.42
00000004408	IPI00570865	94.4%	2.4^-66	Similar to REG4 *	0.38
(G1MZE6)	E1BZV4	94.4%	2.4^-66	Similar to REG4 *	0.38
00000007908	IPI00578482	85.6%	1.8^-121	Similar to α-carbonic anhydrase *	0.35
(G1N6Y5)	E1C004	85.6%	1.8^-121	Similar to α-carbonic anhydrase *	0.35
00000014942	IPI00604279	93.9%	1.8^-120	Clusterin **	0.31
(G1NMV6)	Q9YGP0	93.9%	1.8^-120	Clusterin **	0.31
000000013859	IPI00680520	93.7%	1.9^-210	Similar to pantetheinase/VNN1 *	0.29
(G1NKC5)	E1BUA6	93.7%	1.9^-210	Similar to pantetheinase/VNN1 *	0.29
00000001205	IPI00603434	99.0%	1.7^-137	Dentin matrix protein 4/FAM20C *+	0.27
(G1MSD1)	E1C4X0	99.0%	1.7^-137	Dentin matrix protein 4/FAM20C *+	0.27
00000016580	IPI00583184	97.3%	1.8^-143	Tsukushin *	0.25
(G1NRF2)	TSK	97.3%	1.8^-143	Tsukushin *	0.25
00000010021	IPI00600561	81.2%	0	Chordin *+	0.24
(G1NBK1)	O57465	81.2%	0	Chordin *+	0.24
00000007021	IPI00573727	99.3%	1.2^-115	Similar to nucleobindin-2 **+	0.23
(G1N538)	Q5ZHR1	99.3%	1.2^-115	Similar to nucleobindin-2 **+	0.23
00000008705	IPI00600003	95.2%	1.4^-168	Cathepsin D **+	0.22
(G1N8P0)	CATD	95.2%	1.4^-168	Cathepsin D **+	0.22
00000015671	IPI00573323	90.0%	2.5^-85	Lysozyme G *	0.21
(G1NPK6)	LYG	90.0%	2.5^-85	Lysozyme G *	0.21
00000013696	IPI00585437	89.8%	1.9^-47	Similar to prostate stem cell antigen *	0.18
(G1NJY7)	F1NXM7	89.8%	1.9^-47	Similar to prostate stem cell antigen *	0.18
0000018441	IPI00594564	88.5%	1.1^-133	Similar to nexin-1/serpin E2 **	0.18
(G3URX3)	E1BWU2	88.5%	1.1^-133	Similar to nexin-1/serpin E2 **	0.18
00000009949	IPI00577639	93.9	1.2^-17	Serpin G1/similar to plasma protease C1 inhibitor *	0.16
(G1NBE9)	F1NA58
(G1NBE9)	(partial)
00000007635	IPI00585901	93.9%	1.7^-84	Osteopontin *+	0.15
(G1N6D8)	OSTP	93.9%	1.7^-84	Osteopontin *+	0.15
00000008830	IPI00585604	86.3%	0	Similar to mucin 5AC *	0.15
(G1N8Z1)	E1C037
etc.	etc.
00000010418	IPI00573327	94.2%	1.5^-208	Vitamin D-binding protein **	0.15
(G1NCF1)	Q9W6F5	94.2%	1.5^-208	Vitamin D-binding protein **	0.15
00000003557	IPI00585935	98.0%	0	Similar to semaphorin-3G, fragment *	0.15
(G1MXI6)	F1NQ93	98.0%	0	Similar to semaphorin-3G, fragment *	0.15
00000010338	IPI00598054	93.1%	2.5^-66	Similar to immunoglobin J chain **	0.15
(G1NC90)	E1BY93	93.1%	2.5^-66	Similar to immunoglobin J chain **	0.15
00000003137	IPI00590535	98.4%	0	Fibronectin, fragment **+	0.13
(G1MWJ4)	F1NJT3	98.4%	0	Fibronectin, fragment **+	0.13
00000001542	IPI00587849	94.4%	6.1^-154	Similar to antithrombin/serpin C1 *	0.12
(G1MT40)	F1NLP	94.4%	6.1^-154	Similar to antithrombin/serpin C1 *	0.12
00000002860	IPI00577696	99.0%	0	Uncharacterized protein/similar to glypican-4 **+	0.12
(G1MVZ0)	F1NAU1	99.0%	0	Uncharacterized protein/similar to glypican-4 **+	0.12
00000006152	IPI00574055	99.0%	0	Procollagen-lysine 2-oxoglutarate 5-dioxygenase *	0.12
(G1N380)	PLOD1	99.0%	0	Procollagen-lysine 2-oxoglutarate 5-dioxygenase *	0.12
0000016417	IPI00581002	100%	8.0^-58	Ubiquitin/polyubiquitin **	0.12
(G1NR52)	F1N4V4
(G1NR52)	etc.
00000000900	IPI00595826	99.7%	0	Golgi apparatus protein 1 *+	0.11
(G1MRQ6)	F1P250
(G1MRQ6)	etc.

This table contains all proteins sharing >0.1% of the total proteome. *, indicates previous identification in the chicken eggshell. **, indicates previous identification among the 50 most abundant proteins in the chicken eggshell. For the complete list of identified proteins and details see (Additional file 1: Table S1). +, indicates proteins the message of which was shown to be up-regulated in egg-containing uterus. ¹, the average of two biological replicates (Additional file 1: Table S1). ², immunoglobulins with largely overlapping peptide sets were grouped together. The 47 proteins or protein groups shown in this table represent 95% of the total identified turkey eggshell matrix proteome.

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ISSN: 1477-5956

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