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Table 2 Effect of temperature and fertilizer on accumulation of individual gluten proteins in flour

From: Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86

Predominant protein 1

# Spots

Spot numbers 5

Spot volume × 10 –5 (6)

% Change 11

MN 7

MF 8

HN 9

HF 10

MNvMF

HNvHF

MNvHN

MNvHF

MFvHF

HMW-GS

  

Ave

SD

Ave

SD

Ave

SD

Ave

SD

     

Ax2†

8

12, 16, 17, 18, 19, 23, 24, 28

66.8

2.8

91.3

2.7

91.0

1.2

94.5

6.3

36.7

3.8

36.3

41.6

3.5

Bx7†

8

20, 21, 25, 26, 27, 29, 33, 302

136.3

6.7

170.6

2.7

164.8

2.0

161.6

8.1

25.2

−2.0

21.0

18.6

−5.3

Dx5†

8

3, 5, 7, 10, 14, 36, 307, 538

96.7

0.9

121.6

5.3

128.9

0.2

130.1

5.9

25.7

0.9

33.4

34.6

7.0

By9†

8

40, 47, 52, 349, 350, 351, 358, 360

50.7

1.8

75.6

3.5

77.8

2.4

77.0

5.6

49.2

−1.1

53.6

52.0

1.8

Dy10†

6

42, 48, 51, 333, 424, 514

88.3

6.0

104.7

8.4

105.2

6.0

98.1

10.5

18.6

−6.7

19.1

11.1

−6.3

LMW-GS 2

               

Bu-1 (m-type)

2

167, 170‡

94.9

4.6

99.2

6.2

94.5

4.2

98.1

7.1

4.5

3.8

−0.5

3.3

−1.1

Bu-3† (s-type)

9

119, 119a, 120, 131, 132, 161, 237, 310, 316

282.5

7.5

258.1

7.5

247.1

13.8

237.3

4.8

−8.6

−3.9

−12.5

−16.0

−8.1

Bu-4 (i-type)

3

140‡, 141, 141a

76.8

3.1

82.9

0.5

82.2

7.1

83.0

2.8

8.0

0.9

7.1

8.0

0.0

Bu-6† (m-type)

1

173

35.1

4.1

27.0

2.8

30.5

6.0

26.9

2.4

−23.1

−11.6

−13.1

−23.2

−0.1

Bu-7† (m-type)

3

144, 145‡, 472

102.6

4.6

88.3

5.5

93.5

8.9

87.7

3.2

−14.0

−6.1

−8.9

−14.5

−0.7

Bu-8 (m-type)

1

343

3.4

0.3

2.8

0.1

3.1

0.8

3.3

0.7

−18.7

6.6

−10.1

−4.2

17.9

Bu-11 (m-type)

2

203, 315‡

17.7

1.1

12.2

1.5

11.0

1.3

12.3

1.3

−31.4

11.6

−37.9

−30.7

1.1

Bu-18 (m-type)

2

153‡, 155

40.1

4.3

35.4

3.3

37.1

6.9

33.6

1.9

−11.7

−9.6

−7.4

−16.2

−5.1

Bu-2/13 (s-type)

4

314, 317, 318, 322

92.8

7.7

101.1

3.3

98.2

3.2

90.6

4.9

9.0

−7.7

5.8

−2.3

−10.4

GenBank: AAB48469 (i-type)

1

125

6.5

0.2

6.1

0.4

7.4

0.2

7.7

1.0

−6.3

3.9

14.5

18.9

26.8

TC11-277270 (m-type)

1

319

31.6

3.6

29.8

2.7

35.6

4.2

32.5

1.2

−5.7

−8.7

12.5

2.8

9.0

α-Gliadins

               

Bu-1†

1

342

11.5

0.6

14.7

1.8

12.3

10.6

11.2

1.0

27.9

−9.4

7.6

−2.5

−23.7

Bu-23

2

330, 338

86.8

1.1

97.8

2.9

87.0

1.5

90.4

4.0

12.6

3.9

0.2

4.1

−7.5

Bu-3†

1

468

40.4

1.5

46.7

5.6

42.9

0.5

43.5

3.0

15.6

1.5

6.0

7.6

−6.9

Bu-4†

1

467

53.6

3.1

62.0

2.5

59.9

1.3

58.4

2.7

15.7

−2.5

11.7

8.9

−5.9

Bu-5

2

183, 344

38.6

0.3

46.9

2.0

48.3

3.0

46.5

5.2

21.7

−3.7

25.2

20.5

−1.0

Bu-10

1

550

27.9

1.4

33.8

1.3

31.5

8.0

32.6

3.0

21.5

3.6

13.0

17.1

−3.6

Bu-11

1

327

47.4

4.0

69.6

4.4

67.0

7.6

69.0

4.7

46.7

3.0

41.3

45.5

−0.8

Bu-12†4

5

328, 329, 387, 524, 525

78.6

2.3

118.1

1.9

106.7

10.2

114.7

3.9

50.2

7.5

35.7

45.9

−2.9

Bu-14†

4

190, 206, 331, 551

33.3

0.5

42.5

2.4

45.2

1.6

48.3

6.8

27.5

7.0

35.7

45.2

13.8

Bu-234

1

341

92.0

3.3

97.1

2.4

95.0

5.2

91.6

3.9

5.5

−3.6

3.2

−0.5

−5.7

Bu-27

1

420

14.7

1.4

17.8

2.1

17.5

1.5

16.3

0.9

21.2

−7.2

19.1

10.5

−8.9

Bu (GenBank:BQ806209)

1

177

7.0

0.5

5.9

1.3

5.1

0.7

4.5

0.5

−16.0

−12.2

−27.1

−36.0

−23.7

Bu (GenBank:BQ807130)†

1

124

11.4

1.3

15.3

2.6

16.1

1.7

15.3

0.4

33.9

−5

41.1

34.1

0.1

γ-Gliadins

               

Bu-1†

1

326

4.9

0.4

3.5

0.7

3.9

1.7

3.1

0.6

−28.1

−19.8

−20.5

−36.2

−11.3

Bu-2

1

160

72.4

2.2

80.7

3.0

84.4

3.5

87.9

3.5

11.4

4.2

16.5

21.4

9.0

Bu-43

3

166, 169, 337

92.4

5.2

85.6

8.0

92.7

8.9

84.8

0.4

−7.4

−8.5

0.2

−8.3

−0.9

Bu-5

4

134, 320, 323, 324

142.9

5.1

132.7

6.6

132.9

9.8

127.9

6.9

−7.1

−3.8

−7.0

−10.5

−3.6

Bu-6

3

335, 346, 347

67.4

8.7

72.2

2.4

78.3

6.1

76.2

4.0

7.1

−2.7

16.3

13.1

5.5

Bu-7

1

339

10.6

1.4

9.6

1.1

9.5

0.8

7.8

1.2

−9.7

−17.6

−10.6

−26.4

−18.5

Bu-11

2

163, 527

32.3

1.1

32.2

1.6

36.5

3.3

37.0

0.9

−0.2

1.4

13.1

14.7

15.0

Bu-1 or Bu-83

1

325

31.3

1.5

34.6

1.8

32.9

5.0

32.0

3.8

10.5

−2.8

5.4

2.5

−7.3

ω-Gliadins

               

Gli-B3 type (ω-5)†

6

60, 63, 69, 71, 73, 74

110.9

2.8

230.3

2.6

180.9

20.9

186.1

20.9

107.6

2.9

63.1

67.8

−19.2

Gli-D3 type (ω-1, 2)†

2

476, 477

60.1

3.4

109.4

5.3

108.2

15.5

106.7

15.5

82.0

−1.4

80.1

77.5

−2.4

Bu-D5 (ω-1, 2)†

2

135, 391

25.5

2.5

50.8

4.4

45.7

10.1

49.5

10.1

99.2

8.2

79.5

94.3

−2.5

Cys type TC-262770 (ω-1, 2)†3

4

107, 113, 115, 116

41.2

3.7

64.2

5.1

63.6

9.5

66.6

9.5

56.0

4.6

54.6

61.7

3.6

secalin-like

1

130

3.5

0.5

3.6

0.5

3.1

0.4

3.5

0.4

2.7

13.0

−10.1

1.6

−1.0

  1. 1 Proteins were identified in Dupont et al. [13].
  2. 2 LMW-GS are classified as m-, s- or i-type based on their N-terminal amino acid sequence.
  3. 3 Denotes gliadins containing odd numbers of cysteines.
  4. 4 Denotes α-gliadins that do not contain Glia-α, Glia-α2, Glia-α-9 or Glia-α-20 celiac disease epitopes.
  5. 5 Spots containing same gene sequences.
  6. 6 Ave and SD refer to average and standard deviation of normalized volumes from all spots with the same identification. Additional file 4 contains the data set of normalized spot volumes.
  7. 7 MN, 24/17°C minus fertilizer.
  8. 8 MF, 24/17°C plus fertilizer.
  9. 9 HN, 37/28°C minus fertilizer.
  10. 10 HF, 37/28°C plus fertilizer.
  11. 11Increases of 20% or more are indicated in bold type and decreases of 20% or more are indicated in bold italics type.
  12. † Proteins that responded significantly to fertilizer in Altenbach et al. [14].
  13. ‡ Spot numbers of proteins comprising LMW-GSB, a group of LMW-GS sharing a unique developmental profile.
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