Monitoring post mortem changes in porcine muscle through 2-D DIGE proteome analysis of Longissimus muscle exudate

Table 3 ANOVA (p value), fold changes (calculated from the mean normalised volumes between the groups that showed the maximum change) and average normalised spot volumes of the 40 spots characterised by mass spectrometry

Spot ^a	Anova (p)	Fold change	Mean normalised volumes
			Day 1	Day 3	Day 7
13	0.009	2.2	2.525^a	1.68^ab	1.152^b
15	1.10E-04	2.9	0.338^a	0.881^b	0.965^b
21	7.70E-05	2.3	2.087^a	1.132^b	0.893^b
27	0.013	2.2	0.775^a	0.846^a	1.681^b
35	0.029	2.3	0.512^a	0.832^ab	1.163^b
47	0.004	1.9	0.599^a	1.115^b	1.126^b
56	0.032	1.4	1.585^a	1.135^b	1.144^b
65	0.03	1.3	1.485^a	1.193^b	1.111^b
78	6.96E-04	1.6	1.732^a	1.065^b	1.066^b
86	2.44E-04	2.7	0.215^a	0.581^b	0.47^b
119	0.009	3.4	0.649^a*	1.258^ab	2.213^b*
122	0.005	2.8	0.752^a	0.809^a	2.078^b
124	0.045	2.1	1.386	1.106	0.666
153	0.002	3.4	0.733^a	0.607^a	2.049^b
280	0.021	2.7	0.716^a	1.802^b	1.954^b
358	0.047	2.5	1.525^a	1.028^ab	0.615^b
452	0.044	3.1	0.846^a	0.687^a	2.159^b
462	0.035	1.4	0.866^a	1.227^b	1.125^b#
465	0.046	1.8	0.874^a@	1.532^b@	1.112^ab
552	0.012	2.7	0.745^a	0.762^a	1.99^b
565	0.037	1.3	1.491^a	1.187^b	1.123^b
566	0.023	1.6	1.076^a	1.051^a	1.647^b
591	0.045	1.3	1.649^a	1.388^ab	1.273^b
595	0.005	1.7	1.625^a	1.414^a	0.937^b
648	0.027	2.9	1.019^a&	0.971^a	2.836^b
680	0.004	1.7	1.579^a	1.313^a	0.927^b
962	0.031	1.3	1.048^a	0.866^b	0.818^b
999	5.30E-05	2.2	0.789^a	0.901^a	1.697^b
1007	0.036	1.4	1.46^a	1.076^b	1.114^b
1011	0.007	1.5	1.419^a	1.312^a	0.956^b
1048	0.009	1.4	1.462^a	1.299^a§	1.014^b
1054	0.019	1.3	1.589^a	1.446^ab	1.196^b
1080	0.027	1.3	1.562^a	1.387^ab	1.183^b
1100	8.65E-06	1.8	1.454^a	1.173^b	0.807^c
1135	0.033	1.5	1.066	1.084	1.566
1192	0.004	1.6	1.062^a	1.385^b	1.679^c
1220	0.001	3.1	0.579^a	0.673^a	1.823^b
1245	0.014	1.5	1.339^a	1.216^ab	0.87^b
1291	0.01	1.4	1.315^a	1.276^a	0.944^b
1349	0.044	1.2	1.405^a◄	1.264^ab	1.138^b◄

Within rows, different superscripts indicate significantly different means at the 5% level (following Tukey-Kramer post hoc analysis). * indicates that p value was 0.061. # indicates that p value was 0.053. @ indicates that p value was 0.058. & indicate that p value was 0.056. § indicate that p value was 0.062. ◄ indicate that p value was 0.054. ^aSpot numbers refer to Figure 1.

ISSN: 1477-5956