Figure 4
From: PhosSA: Fast and accurate phosphorylation site assignment algorithm for mass spectrometry data
Sensitivity and Accuracy for the 6 samples are shown with varying (dCn) threshold: A shows the accuracy and B shows the sensitivity for CID samples. AQP2-H-(S256/S261), AQP2-M-(S256/S261) and AQP2-L-(S256/S261) consists of phosphopeptide standard (Biotin-LC-CEPDTDWEEREVRRRQS*VELHS*PQSLPRGSKA) phosphorylated at both S 256 and S 261 in distinct amounts of 0.2 nmol, 20 pmol and 2 pmol respectively. AQP2-H-(S264), AQP2-M-(S264) and AQP2-L-(S264) consists of peptide standard (Biotin-LC-CEPDTDWEEREVRRRQSVELHSPQS*LPRGSKA) phosphorylated at S 264 in amounts of 0.2 nmol, 20 pmol and 2 pmol respectively.