Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine

Table 3 Proteomic analysis of Gβ subunits isolated from brain cortex of control and morphine-treated rats

Spot	Accession	Protein name	Mascot	Matched	Peptides	SC^a	MW^b	pI^c
	number		score	peptides		[%]	(kDa)
3	GBB1_RAT	Guanine nucleotide-binding protein	184.3	6	R.LFDLR.A	16.8	37.4	5.6
		subunit beta-1			R.LLVSASQDGK.L
					K.LWDVR.E
					K.IYAMHWGTDSR.L + Oxidation (M)
					K.ACADATLSQITNNIDPVGR.I
					K.VHAIPLR.S
4	GBB1_RAT	Guanine nucleotide-binding protein	471	12	K.ACADATLSQITNNIDPVGR.I	32.9	37.4	5.6
		subunit beta-1			R.LFVSGACDASAK.L
					K.IYAMHWGTDSR.L + Oxidation (M)
					R.LFDLR.A
					K.IYAMHWGTDSR.L
					R.LLVSASQDGK.L
					K.LWDVR.E
					R.KACADATLSQITNNIDPVGR.I
					R.LLLAGYDDFNCNVWDALK.A
					K.VHAIPLR.S
					K.LIIWDSYTTNK.V
					R.ELAGHTGYLSCCR.F
4	GBB2_RAT	Guanine nucleotide-binding protein	413.9	10	R.TFVSGACDASIK.L	14.4	37.3	5.6
		subunit beta-2			K.ACGDSTLTQITAGLDPVGR.I
					K.IYAMHWGTDSR.L + Oxidation (M)
					R.LFDLR.A
					K.IYAMHWGTDSR.L
					R.LLVSASQDGK.L
					K.LWDVR.D
					K.VHAIPLR.S
					K.LIIWDSYTTNK.V
					R.LLLAGYDDFNCNIWDAMK.G + Oxidation (W)
5	GBB1_RAT	Guanine nucleotide-binding protein	199.1	5	R.AGVLAGHDNR.V	14.1	37.4	5.6
		subunit beta-1			R.LFVSGACDASAK.L
					R.LFDLR.A
					K.IYAMHWGTDSR.L + Oxidation (M)
					R.LLVSASQDGK.L
5	GBB2_RAT	Guanine nucleotide-binding protein	188.7	5	R.AGVLAGHDNR.V	3.5	37.3	5.6
		subunit beta-2			R.TFVSGACDASIK.L
					R.LFDLR.A
					K.IYAMHWGTDSR.L + Oxidation (M)
					R.LLVSASQDGK.L
7	GBB (1–4)_RAT	Guanine nucleotide-binding protein	22.4	1	K.LLVSASQDGK.L	2.9	37.2	5.4
		subunit beta-3
8	GBB (1–4)_RAT	Guanine nucleotide-binding protein	34.7	1	K.LLVSASQDGK.L	2.9	37.2	5.4
		subunit beta-3

ISSN: 1477-5956