Table 2 The proteins identified by proteomics analysis
| Â | Name | Accession number | Protein score | Mass | pI | Peptides identified | % Peptide coverage | rhBMP-2 induction/Control |
|---|---|---|---|---|---|---|---|---|
1 | Eukaryotic translation initiation factor 3 subunit K isoform 1 | gi|57036583 | 95 | 25342.6 | 4.81 | YNPENLATLER | 25.7 | 0.3 |
ALTNLPHTDFTLCK | ||||||||
GIDRYNPENLATLER | ||||||||
CMIDQAHQEERPIR | ||||||||
2 | Heat shock protein beta-6 | gi|57038366 | 100 | 17382.1 | 5.95 | HFSPEEIAVK | 21 | <0.1 |
VVGDHVEVHAR | ||||||||
HEERPDEHGYIAR | ||||||||
3 | Annexin A8 isoform 1 | gi|73953207 | 102 | 36827.5 | 5.53 | VFEEYER | 39 | <0.1 |
FITILCTR | ||||||||
NLHSYFAER | ||||||||
ILVCLLQGSR | ||||||||
EGVIIEILASR | ||||||||
GIGTNEQAIIDVLTR | ||||||||
DLTETLQSELSGKFER | ||||||||
NLHSYFAERLYYALK | ||||||||
GSPHFNPEPDAEALYTAMK | ||||||||
AQFGKDLTETLQSELSGK | ||||||||
4 | LIM and SH3 domain protein 1 isoform 2 | gi|73966152 | 89 | 30162.4 | 6.33 | EPAAPVSIPR | 38 | <0.1 |
QQSELQSQVR | ||||||||
IPYCNAHYPK | ||||||||
GFSVVADTPELQR | ||||||||
LKQQSELQSQVR | ||||||||
GKGFSVVADTPELQR | ||||||||
QSFTMVADTPENLR | ||||||||
TQDQISNIKYHEEFEK | ||||||||
5 | Glucose-6-phosphate isomerase | gi|296080693 | 94 | 63147 | 8.42 | VDHQTGPIVWG | 7.5 | <0.1 |
KTFTTQETIT | ||||||||
RSGDWKGYTGCETHAMLP YDQ | ||||||||
6 | RAD23 homolog B | gi|57094213 | 99 | 43171 | 4.77 | A PTPVPALAPT | 10.6 | <0.1 |
QAYFACEKNEN | ||||||||
AAKQEKPAEK | ||||||||
VVMVTKPKAVS | ||||||||
7 | Fibronectin 1 | gi|345797318 | 99 | 262625 | 5.46 | LEPGTEYTI | 3 | <0.1 |
RARIAGYRLT | ||||||||
CSTTSNYEQDQ | ||||||||
STSQTTAPDAP | ||||||||
YVVGETWEKPYQG | ||||||||
LSISPSDNVVVLTNLL | ||||||||
8 | Poly(rC) binding protein 1 | gi|222352151 | 95 | 37498 | 6.66 | SSSPEVKGYWA | 7 | <0.1 |
NSSMTNSTAASRPP | ||||||||
9 | Signal transducer and activator of transcription 1 | gi|74005006 | 68 | 87335 | 5.74 | FHAVEPYTK | 6 | <0.1 |
LWYNMLVTE | ||||||||
RQQSACIGGPPN | ||||||||
VIDLETTSLPVVV | ||||||||
10 | Retinol-binding protein 4 | gi|73998292 | 100 | 30177 | 8.48 | QEELCLAR | 49 | >10 |
RLPSVFHPGR | ||||||||
DPNGLPLEAQK | ||||||||
QRQEELCLAR | ||||||||
LIVHNGYCDGR | ||||||||
LIVHNGYCDGRSEPNTL | ||||||||
LLNLDGTCADSYSFVFSR | ||||||||
KDPEGLFLQDNIVAEFSVDENGR | ||||||||
GNDDHWIIDTDYDTYAVQYSCR | ||||||||
11 | Ferritin light chain | gi|66864897 | 100 | 20139.2 | 5.66 | MGDHLTNLRR | 73 | 4 |
ELAEEKREGAER | ||||||||
DDVALEGVGHFFR | ||||||||
QNYSTEVEAAVNR | ||||||||
LATPQAGLGEYLFER | ||||||||
SLNQALLDLHALGSAR | ||||||||
ASYTYLSLGFYFDR | ||||||||
RLATPQAGLGEYLFER | ||||||||
ADPHLCDFLENHFLDEEVK | ||||||||
12 | Ferritin heavy chain | gi|302393573 | 100 | 21408.4 | 5.53 | ELGDHVTNLR | 66 | 3 |
SVNQSLLELHK | ||||||||
YFLHQSHEER | ||||||||
IFLQDIKKPDR | ||||||||
SIKELGDHVTNLR | ||||||||
QNYHQDSEAAINR | ||||||||
SIKELGDHVTNLRK | ||||||||
YFLHQSHEEREHAEK | ||||||||
LATDKNDPHLCDFIETHYLNEQVK | ||||||||
13 | Annexin A4 | gi|55742853 | 96 | 36075.2 | 5.72 | VLVSLSAGGR | 60 | >10 |
FLTVLCSR | ||||||||
NKSAYFAER | ||||||||
SDTSFMFQR | ||||||||
DEGNFLDDALMR | ||||||||
NHLLHVFDEYKR | ||||||||
NRNHLLHVFDEYK | ||||||||
SLYSFIKGDTSGDYR | ||||||||
AEIDMMDIRESFKR | ||||||||
SLYSFIKGDTSGDYRK | ||||||||
NRNHLLHVFDEYKR | ||||||||
DLMDDLKSELSGNFER | ||||||||
GGTVKPASGFSATEDAQTLR | ||||||||
VLVSLSAGGRDEGNFLDDALMR | ||||||||
14 | Proteasome activator complex subunit 3, isoform 6 | gi|73965646 | 84 | 29601.6 | 5.69 | YPHVEDYR | 45 | 3 |
LKVDSFRER | ||||||||
YPHVEDYRR | ||||||||
IAKYPHVEDYRR | ||||||||
RLDECEEAFQGTK | ||||||||
TVESEAASYLDQISR | ||||||||
ITSEAEDLVANFFPK | ||||||||
TVTEIDEKEYISLR | ||||||||
IEDGNNFGVSIQEETVAELR | ||||||||
15 | Adenosylhomocysteinase isoform 1 | gi|73991635 | 100 | 48260.4 | 5.98 | RIILLAEGR | 14.8 | 3 |
FDNLYGCR | ||||||||
YPQLLSGIR | ||||||||
VNIKPQVDR | ||||||||
SKFDNLYGCR | ||||||||
VAVVAGYGDVGKGCAQALR | ||||||||
16 | Histidyl-tRNA synthetase, cytoplasmic, isoform 1 | gi|73949318 | 73 | 57880.3 | 5.44 | IFSIVEQR | 8 | >10 |
YDLTVPFAR | ||||||||
DQGGELLSLR | ||||||||
KVPCVGLSIGVER | ||||||||
17 | Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3 | gi|119618005 | 87 | 40,095 | 9.45 | SVVSVLNKE | 10 | 4 |
DAAPKMYKEEGL | ||||||||
IFNGFSVTLKEDGVRG | ||||||||
18 | ATP-binding cassette, sub-family B (MDR/TAP), member 1 | gi| 51094928 | 101 | 141479 | 9.06 | EGLMPNTL | 6 | 3 |
LSLEVKKG | ||||||||
AVKEANAYDFI | ||||||||
GLQPAFAIIFSKIIG | ||||||||
LTLVILAISPVLGLS | ||||||||
AGNLEDLMSNITNRSDIND | ||||||||
19 | DEAD (Asp-Glu-Ala-Asp) box helicase 1 | gi| 148666036 | 66 | 82432 | 6.80 | ERLGKNHI | 7 | >4 |
CLHGDRKPHERKQN | ||||||||
GCYLDIDKGHVKFSK | ||||||||
AIGSDGLCCQSREVKE | ||||||||
20 | Isocitrate dehydrogenase 1 (NADP+), soluble | gi|119590847 | 99 | 46659 | 6.53 | EVSIETIEA | 11 | 3 |
SPNGTIRNI | ||||||||
GTQKVTYLVHNF | ||||||||
DDMVAQAMKSEGGFI | ||||||||
21 | Filamin B, beta | gi| 410336835 | 73 | 278164 | 5.47 | LEFLDRES | 2.5 | 3 |
EAGEGDVSV | ||||||||
PHVVKVFFAG | ||||||||
ECSDNGDGTCS | ||||||||
ILVKYNDKHIPGS | ||||||||
KQPAKFTVDTISAG |