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Figure 2 | Proteome Science

Figure 2

From: Metabolic labeling with stable isotope nitrogen (15N) to follow amino acid and protein turnover of three plastid proteins in Chlamydomonas reinhardtii

Figure 2

Spectrum of Leu when grown in TAP (A) or in 15 N-TAP (B). Free amino acids isolated from algae subcultured three times in fresh TAP or in fresh 15N-TAP medium were analyzed by GC-MS. (A) The major fragment ions for 14N-Leu circled in red are 144,115,102 and 88 (m/z). (B) The spectrum for 15N-Leu, from algae grown in 15N-TAP, display a shift of each fragment ion peak by +1 corresponding to the replacement of 14N to 15N; peaks 145, 116, 103 and 89; circled in red on the figure.

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ISSN: 1477-5956