Figure 1

Two-dimensional gel electrophoresis of bovine milk fractions. Each gel was loaded with 350 μg of protein from whey (A, B), milk fat globule membrane (C, D) or basic protein fraction (E, F) from either pooled milk from the infected quarters taken before inoculation (A, C, E) or from the same quarters taken at the time of diagnosis of clinical mastitis (B, D, F). The gels were stained with Coomassie blue G250. The spots that were consistently altered greater than two-fold in abundance between the uninfected and mastitis gels, and that were identified as a host-defence related protein, are indicated. The number of each spot corresponds to that in the wider set of 240 spots subjected to MALDI-TOF analysis and listed in Additional file 2 and shown in Additional file 1.