Figure 3

Western blotting of individual milk samples. Portions of each milk sample were diluted 15-fold in sample buffer and subjected to SDS electrophoresis followed by western blotting using antibodies raised against lactoferrin (LF), the cathelin domain of cathelicidin-1 (Cath), CG39, S100A9, S100A12, secretory component (SC), and RNase5. Peroxidise-conjugated goat anti-rabbit IgG was used as secondary antibody. The signals were visualised by enhanced chemiluminescence followed by exposure to X-ray film. Quarters for each cow were untreated (1), mock inoculated (2), or infused with S. uberis (3 and 4). A replicate gel was stained with Coomassie blue (bottom panel).