Host defence related responses in bovine milk during an experimentally induced Streptococcus uberis infection

Smolenski, Grant A; Broadhurst, Marita K; Stelwagen, Kerst; Haigh, Brendan J; Wheeler, Thomas T

doi:10.1186/1477-5956-12-19

Proteome Science

Table 1 List of 53 proteins identified by 2DE-MALDI with mean abundance altered at least two-fold with mastitis

From: Host defence related responses in bovine milk during an experimentally induced Streptococcus uberis infection

Identified protein	Accession no.	Spot no.	Function	Fold change	p-value^†	Fraction
Actin, beta	AAI42414	104	Structural	3.04	0.040	MFGM
Actin, cytoplasmic 2	NP_001028790	130	Structural	20.93	0.054	MFGM
Adipose differentiation-related protein	ABM06155	106	Unknown	0.08	0.119	MFGM
Alpha-lactalbumin	CAA44927	110	Major milk protein	5.79	0.158	MFGM
Alpha-S1 casein	AAA30429	214	Major milk protein	0.16	0.034	BF
Alpha-S2 casein	CAH61065	219	Major milk protein	207.0	0.002	BF
Annexin A1	CAA39971	134	Structural	28.8	0.020	MFGM
Annexin A2	NP_777141	133	Structural	14.76	0.027	MFGM
Annexin A5	NP_001035567	138	Structural	5.16	0.116	MFGM
Apolipoprotein A-I	NP_776667	147	Host defence	8.12	0.144	MFGM
Beta casein	AAA30431	226	Major milk protein	18.0	0.003	BF
Beta-2-microglobulin	NP_776318	31	Host defence	0.06	0.006	Whey
Beta-lactoglobulin	732164A	109	Major milk protein	4.4	0.005	MFGM
Butyrophilin subfamily 1 member A1	P18892	96	Unknown	0.33	0.282	MFGM
Cathelicidin-1	DAA16881	220	Host defence	8.0	0.001	BF
Cathelicidin-7*	NP_777256	158	Host defence	37.54	0.023	MFGM
Chitinase-3-like protein-1	AAB64304	58	Host defence	47.8	0.019	Whey
Complement component C3	DAA27847	177	Host defence	0.004	<0.001	BF
Complement component C7 precursor	DAA17890	164	Host defence	0.41	0.052	BF
Endopin 1A	Q9TTE1	38	Metabolism	6.35	0.072	Whey
Enolase-1*	NP_776474	49	Metabolism	0.28	0.005	Whey
Fibrinogen	NP_001136389	55	Structural	0.09	0.028	Whey
Folate binding protein	1011184A	180	Metabolism	0.001	<0.001	BF
Gelsolin isoform b	NP_001029799	167	Host defence	0.24	0.002	BF
Glycosylation-dependent cell adhesion molecule-1	NP_777253	115	Host defence	0.45	0.047	MFGM
Haptoglobin	NP_001035560	65	Metabolism	74.1	0.020	Whey
Heat shock 70 kDa protein 5 (GRP 78)*	NP_001068616	117	Chaparonin	3.62	0.109	MFGM
Heat shock cognate 71 kDa protein	NP_776770	119	Chaparonin	7.58	0.060	MFGM
HRPE773	DAA15703	224	Unknown	8.09	0.003	BF
IgG₁ heavy chain constant region	ABE68619	16	Host defence	3.6	0.353	Whey
IgM heavy chain constant region	AAB62251	87	Host defence	0.42	0.245	MFGM
Kappa casein	AF123251	183	Major milk protein	0.06	0.001	BF
Keratin, type II cytoskeletal 2 epidermal	XP_001254016	204	Structural	0.48	0.029	BF
Lactadherin (glycoprotein antigen MGP57/53)	S65138	97	Unknown	0.31	0.176	MFGM
Lactoferrin	AAA30610	48	Host defence	4.97	0.040	Whey
Lactoperoxidase	NP_776358	197	Host defence	3.07	0.010	BF
Pancreatic adenocarcinoma upregulated factor	DAA15702	236	Host defence	2.75	0.003	BF
Peptidoglycan recognition protein-1	NP_776998	239	Host defence	10.2	0.157	BF
Pigment epithelium-derived factor	NP_776565	174	Metabolism	0.51	0.013	BF
Polymeric immunoglobulin receptor	NP_776568	165	Host defence	0.05	0.002	BF
Quiescin Q6 sulfhydryl oxidase-1	NP_001095544	209	Metabolism	0.29	0.030	BF
S100 calcium-binding protein A12	P79105	81	Host defence	27.3	0.029	Whey
S100 calcium-binding protein A8	NP_001107197	160	Host defence	90.0	0.092	MFGM
S100 calcium-binding protein A9	NP_001039793	151	Host defence	9.14	0.044	MFGM
Serotransferrin	NP_803450	7	Transport	2.0	0.121	Whey
Serpin B1	Q1JPB0	52	Metabolism	0.47	0.013	Whey
Serpin B4*	NP_001095792	54	Metabolism	0.15	0.029	Whey
Serum albumin	NP_851335	172	Transport	6.11	0.022	BF
Serum amyloid A3	NP_851359	159	Host defence	10.03	0.046	MFGM
Serum amyloid A3.2 precursor*	XP_875660	76	Host defence	812.0	<0.001	Whey
Similar to serpin peptidase inhibitor, clade B like*	XP_001254097	57	Metabolism	0.31	0.261	Whey
Xanthine dehydrogenase	NP_776397	94	Host defence	0.12	0.038	MFGM
Zymogen granule membrane GP-2	NP_001069418	105	Host defence	0.4	0.072	MFGM

*denotes detection only by 2DE/MALDI-TOF.
^†Results of the adjusted Students T test for significance of the difference from the three replicate gels per condition. Values in bold type are below 0.05, and those in italics are between 0.05 and 0.10.

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ISSN: 1477-5956

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